肉苁蓉多糖SPA结构的GC-MS及^(13)C-NMR分析  

GC-MS and ^(13)C-NMR analysis on studying polysaccharide SPA structure

作　　者：赵伟[1] 颜宏[2] 梁忠岩[2] 

机构地区：[1]空军航空大学航空救生系,吉林长春130022 [2]东北师范大学生命科学学院,吉林长春130024

出　　处：《分子科学学报》2005年第2期36-39,共4页Journal of Molecular Science

基　　金：国家自然科学基金资助项目(39870847)

摘　　要：从野生肉苁蓉茎中提取水溶性多糖,经Seveg法和酶法脱蛋白,超滤分级及SephadexG75制备柱分级,得均一组份SPA.经甲基化及GC/MS,13CNMR分析证明SPA由(1→6)Glc组成主链,且Glc残基在3O处有一分枝,侧链由(1→4)Gal,(1→4)Man,(1→3)Gal及(1→2)Rha组成,由(1→)Ara构成侧链末端.A water soluble crude polysaccharide has been isolated from the stem of the wild Cistanche deserticola Ma. It was deproteinized by Sevage method and proteinase method, was fractionated byultrafiltration and Sephadex G-75 column chromatography .A polysaccharide fraction SPA was collected. By means of mathylation analysis, GC-MS and ^(13)C-NMR, the results revealed the main chain was made up of (1→6)-linked-Glc residues and substituted at 3-O. The side chains is composed of (1→4)-linked-Gal, (1→4)-linked-Man, (1→3)-linked-Gal and (1→2)-linked-Rha residues. The nonreduced end is composed of (1→)Ara.

关 键 词：肉苁蓉 多糖 分析方法 Seveg法 酶法 植物 核磁共振技术 

分 类 号：Q539[生物学—生物化学] Q949.778.3