贲门癌及其癌前病变中细胞增殖与细胞凋亡的比较和研究  

Research and Compare on the Cellproliferation and Apoptosis bwtween Cardiac Carcinoma and its Precancerous Lesions

作　　者：王少洪[1] 沈金辉[1] 方可君[1] 周厚强[1] 曾绍文[1] 

机构地区：[1]汕头市中心医院,广东汕头515031

出　　处：《基层医学论坛》2005年第4期293-295,共3页The Medical Forum

基　　金：(本课题为汕头市重点科技计划项目编号200104)

摘　　要：目的探讨细胞增殖与细胞凋亡在贲门癌发生发展中的作用。方法应用免疫组化法检测PC N A、p53、Bcl-2在30例贲门癌、10例胃溃疡胃壁组织中的表达和分布,采用病理图像分析的方法进行灰度检测,并对胃壁上皮组织或癌组织中凋亡细胞进行计数。结果观察和比较自胃远端切缘、癌旁至癌灶等胃壁组织中的变化,发现PC N A呈显著的增高趋势,癌灶组织中PC N A呈强阳性表达;p53在正常胃黏膜组织中不表达,在癌灶组织中呈强阳性表达;Bcl-2在癌旁组织中呈强阳性表达,显著高于癌灶组织;细胞凋亡指数在癌灶组织中显著降低。胃溃疡病灶边缘的胃黏膜组织PC N A呈阳性表达,p53呈阴性表达,Bc1-2呈弱阳性表达,细胞凋亡指数显著增高。结论在非典型增生的贲门黏膜组织中,细胞增殖和细胞凋亡均非常活跃,而一旦细胞凋亡受到严重抑制,即可导致贲门癌的发生。Objective To investigate the role of cellproliferation and apoptosis on the carcinogenesis and development in cardiac carcinoma. Methods With immunohistochemistry stain,it was measured the distribution and expression of PCNA,p53 and Bcl-2 in the gastric tissues of the cardiac carcinoma(n=30), gastric ulcer(n=10) and measured their gray scales with the method of the pathomorphological analysis. The number of apoptosis cell was accounted in the gastric epithelial tissue or cardiac carcinoma tissue. Results The expressive level of PCNA gradually increased in the tissues from the gastric far-end cutting edge, peripheral to center areas of cardiac carcinoma and the expression was fortis positive in the carcinoma tissue;The expression of p53 was negative in the normal gastric mucous membrane and fortis positive in the carcinoma tissue; the expression of Bcl-2 was fortis positive in the peripheral area of the carcinoma tissue and it was noticeable higher than that in the carcinoma tissue. Apoptosis index (AI) was noticeably decreased in the carcinoma tissue. In the gastric mucous membrane of the gastric ulcer focus edge, the expression of PCNA and p53was positive; the expression of Bcl-2 was low positive; AI noticeably increased. Conclusion Both of the cellproliferation and apoptosis were very active in the atypical hyperplasis cardiac mucous membrane. If the mechanism of the apoptosis was highly restrained, the carcinogenesis of cardiac carcinoma was induced in the cardiac mucous membrane.

关 键 词：细胞增殖 贲门癌 癌前病变 Bcl-2 免疫组化法检测 阳性表达 胃黏膜组织 PCNA 病理图像分析 细胞凋亡指数 胃壁组织 非典型增生 p53 发生发展 方法应用 凋亡细胞 上皮组织 结果观察 癌旁组织 溃疡病灶 阴性表达 癌灶 胃溃疡 

分 类 号：R735.2[医药卫生—肿瘤]