PEX基因对肿瘤细胞生物学性状的影响  

Effects of PEX gene on the tumor cytobiological characters

作　　者：许传杰[1] 王贵全[2] 张立红[3] 朱桂彬[3] 王英[3] 李玉林[3] 

机构地区：[1]吉林大学第二医院病理科,吉林长春130041 [2]吉林大学第二医院信息中心 [3]吉林大学病理生物学教育部重点实验室

出　　处：《中国实验诊断学》2005年第2期178-181,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　探讨PEX基因在体外对鼠黑色素瘤细胞B16F10生物学性状的影响。方法　利用脂质体介导转染法,经G4 18稳定筛选后,应用MTT、流式细胞术、软琼脂克隆形成实验及Boyden小室侵袭实验,体外观察PEX基因对B16F10细胞生物学性状的影响。结果　BpcDNA sPEX、BpcDNA、B16F10三组细胞生长速度、细胞周期各时相均无明显差异(P >0 . 0 5 ) ;BpcDNA sPEX组细胞集落与BpcDNA组和B16F10组相比统计学上存在显著性差异(P <0 . 0 1) ,BpcD NA sPEX组细胞集落比BpcDNA组和B16F10组少,且单个集落中细胞数目少;BpcDNA sPEX组细胞穿透Matrigel胶能力比BpcDNA和B16F10组细胞弱,统计学上存在显著差异(P <0 . 0 1)。结论　PEX基因转染对体外单纯培养的B16F10细胞生长、增殖无影响;PEX基因可抑制B16F10细胞在软琼脂中形成集落的能力及体外侵袭能力。Objective To study the effects of PEX gene on the biological characters of mouse melanoma cells(B16F10) in vitro. Methods To use liposome-mediated transfection, G418 stable screening, MTT, flow cytometry, soft agarose cloning technique and Boyden cabin invasive technique to observe the effects of PEX gene on B16F10 cytobiological characters.Results There was no difference of cells growth speed and every phase of cells cycle amony the three groups of BpcDNA-sPEX, BpcDNA and B16F10 ( P >0.05);There was significant difference of cells colonies between BpcDNA-sPEX group and BpcDNA group and B16F10 group( P <0.01), The cells colonies of BpcDNA-sPEX group were smaller than those of BpcDNA group and B16F10 group, and the number of cells in one colony was also less. The Matrigel-gel penetration ability of cells in BpcDNA-sPEX group was lower than that in BpcDNA group and B16F10 group, and there was statistical difference( P <0.01).Conclusion PEX gene transfection had no effect on the cells growth and proliferation of purely-cultured B16F10 in vitro. PEX gene can repress colony-forming ability in soft agarose and invasive ability in vitro of B16F10 cells.

关 键 词：PEX基因 真核表达载体 鼠黑色素瘤细胞 细胞外基质 

分 类 号：R73-3[医药卫生—肿瘤]