深度学科交叉和高度过程集成的非水相生物催化  

Multidiscipline and process integration of nonaqueous biocatalysis

作　　者：王志龙[1] 

机构地区：[1]上海交通大学药学院,上海200030

出　　处：《化工学报》2005年第4期563-569,共7页CIESC Journal

摘　　要：和化学合成相比, 生物催化具有选择性高、反应条件温和、环境友好的特性而成为生物技术中一个快速发展的领域. 但生物催化工业常受到底物溶解度低、底物/产物抑制生物催化剂活性、产物进一步降解等限制.非水相催化和原位产物去除新技术是克服上述困难的有力工具, 本文综述了其研究进展. 以甾醇边链切除的微生物转化为例, 介绍了作者最近开发的高度集成了非水相催化和原位产物去除优点的浊点两相分配生物反应器技术. 讨论了非水相生物催化工业高度过程集成和深度学科交叉的特征, 提出了发展非水相生物催化工业的若干建议.When compared to their chemical counterparts, the peculiar properties of biocatalysts, such as high selectivity, reaction under mild conditions and environmental benefit, have made biocatalysis a broad and growing field of biotechnology. It is often constrained by some obstacles, such as limited substrate accessibility to biocatalysts because of the low aqueous solubility of most organics, inhibition or toxicity of both substrate and product affecting the biocatalysts, and further degradation of products. Biocatalysis in nonaqueous solvent and in situ product removal are powerful tools to overcome these limits. The development of these techniques is reviewed. The microbial transformation of sterol in the cloud point system, a novel two-phase partitioning bioreactor developed recently by the authors, as an example of highly integrated process of biocatalysis in nonaqueous solvent and in situ product removal techniques is introduced. Based on the discussion of nonaqueous biocatalysis industry involving the characteristics of integrated process and multidisciplinary, nature of the development of nonaqueous biocatalysis industry is recommended.

关 键 词：非水相生物催化 原位产物去除 两相分配生物反应器 浊点系统 工程放大 

分 类 号：TQ923[轻工技术与工程—发酵工程]