碳源、氮源及无机盐对粉拟青霉生长的影响  

The Influence of Carbonaceous Compounds, Nitrogenous Substances and Inorganic Matter on the Mycelia Biomass of Paecilomyces farinosus Cultured in Liquid Media

作　　者：杨斌[1] 柳成益[1] 姚金鹏[1] 吴天龙[1] 

机构地区：[1]西南林学院资源学院,云南昆明650224

出　　处：《西南林学院学报》2005年第1期9-12,共4页Journal of Southwest Forestry College

基　　金：云南省攻关项目(2002NG04);云南省林业厅(2003092);昆明市科技局资助项目(200243).

摘　　要：采用液体发酵方法,在25 ℃条件下,实验了真菌培养常见的9种碳源物质,7种氮源物质、6种无机盐对粉拟青霉菌丝生长的影响.结果表明,玉米粉、蔗糖、麦芽糖和葡萄糖是粉拟青霉菌丝生长较好的碳源物质,采用这几种碳源液体培养72 h后干菌丝产量可达7.33～8.04 mg/mL;牛肉膏蛋白胨是较好的氮源物质,72 h干菌丝产量为8.87 mg/mL,是其他氮源的3.5～6.0倍.磷酸二氢钾较适合该菌生长,72 h干菌丝产量为7.62 mg/mL,硫酸亚铁和硫酸钙不是菌丝生长的最适无机盐,但这两种盐有利于该菌在液体状态下产生节孢子,节孢子浓度可达108个/mL.The entomopathogenic fungal isolate (Paecilomyces farinosus) sw 03085 was cultured in submerged condition at 25 using 9 hydrocarbons, 7 nitrogenous compounds and inorganic matters. Based on the production of dry mycelia in incubation time, four hydrocarbons (corn flour, saccharose, maltose, dextrose) were selected for using in mass liquid ferment, and in 72 h dry mycelia production could reach 7.338.04 mg/mL. The dry mycelia biomass in Beef peptone media could reach 8.87 mg/mL in 72 h that was 3.56.0 times than the production in other nitrogenous substance media. Among several experimental inorganic matters, KH_2PO_4 was the most fitful inorganic salt and the mycelia biomass could be 7.62 mg/mL in 72 h. The experimental result also indicated FeSO_4 and CaSO_4 benefit to the production of nodal spores of the fungus and spore concentration could be 10~8/mL.

关 键 词：粉拟青霉 菌丝生物量 碳源 氮源 无机盐 生长 液体发酵 昆虫病原菌 微生物杀虫剂 

分 类 号：S767.32[农业科学—森林保护学]