HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method  

作　　者：ZeYiYAN ChengJinJIAO FengMinLI YongMinLIANG ZhiXiaoLI 

机构地区：[1]StateKeyLaboratoryofAppliedOrganicChemistry,LanzhouUniversity,Lanzhou730000 [2]SchoolofLifeScience,LanzhouUniversity,Lanzhou730000

出　　处：《Chinese Chemical Letters》2005年第5期639-642,共4页中国化学快报（英文版）

基　　金：This work was supported by NKBRSF Project(G2000018603);the National Natural Science Foundation of China(No.30270965).

摘　　要：An assay is presented for simultaneously determining 5 biogenic amines and the major non-protein amino acids: the toxin β-N-oxalyl-L-α,β-diaminopropanoic acid (β-ODAP), its isomer α-ODAP and homoarginine in Lathyrus sativus extracts using the HPLC system after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-Cl). However, it is more worthy of noting that this paper also describes a new extraction method using 0.2 mol/L HClO4. The new method has some advantages: shorter extraction-time, simultaneous extraction of free amino acids and polyamines, better inhibiting the isomerization of β-ODAP to α-ODAP, and so on.

关 键 词：ODAP homoginine HPLC biogenic amines new extraction method. 

分 类 号：Q949.751.9[生物学—植物学]