小檗碱对人胃癌细胞株BGC-823诱导凋亡的研究  

The study apoptosis of human gastric carcinoma BGC-823 cell induced by berberine

作　　者：李国英[1] 杨林西[2] 王玉平[2] 

机构地区：[1]甘肃省红十字血液中心,兰州730046 [2]兰州医学院生物化学与分子生物学教研室,兰州730000

出　　处：《中药药理与临床》2005年第1期16-19,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:研究小檗碱对人胃癌BGC- 82 3细胞诱导凋亡的作用。方法:应用荧光显微镜、透射电镜、流式细胞仪和TUNEL染色研究小檗碱对人胃癌BGC- 82 3细胞诱导凋亡的作用。结果:小檗碱作用人胃癌BGC -82 3细胞后,可看到较为典型的细胞凋亡的形态学变化,细胞核固缩、染色质凝集成新月状紧贴于核膜周边,核碎裂、染色质片断化、凋亡小体形成等。流式细胞仪上出现典型的亚二倍体凋亡峰。TUNEL染色显示,随小檗碱浓度增加和作用时间延长,细胞凋亡率增加。结论:小檗碱对人胃癌BGC- 82 3细胞有诱导凋亡作用。Objective: To study the effects of berberine on human gastric carcinoma BGC-823 cell in induction of apoptosis. Methods: The apoptosis of human gastric carcinoma BGC-823 cell was studied by fluorescent microscopy, electronic microscopy, flow cytometry and TUNEL method after treatment with berberine. Results: After exposure to berberine, the human gastric carcinoma BGC-823 cell presented some morphologic features of apoptosis, including cell shrinkage, nuclear condensation, DNA fragmentation and apoptotic bodies formation. Some typical subdiploid peaks before G0/G1 phase were observed through flow cytometry. Along with berberine increased and prolonged, the apoptosis of human gastric carcinoma BGC-823 cell was raised after treatment with berberine. Conclusions: Berberine was able to induce the apoptosis in human gastric carcinoma BGC-823 cell.

关 键 词：小檗碱 人胃癌细胞株 BGC-823细胞 TUNEL染色 流式细胞仪 诱导凋亡作用 荧光显微镜 形态学变化 细胞凋亡率 透射电镜 染色研究 亚二倍体 时间延长 核固缩 核碎裂 染色质 

分 类 号：R735.2[医药卫生—肿瘤]