端粒酶在乳腺癌中的表达研究  

Breast cancer and telomerase activity

作　　者：刘法文[1] 张静[1] 张恒伟[1] 

机构地区：[1]河南省肿瘤医院肿瘤研究所,郑州市450008

出　　处：《中原医刊》2005年第9期1-2,共2页Central Plains Medical Journal

基　　金：河南省科技攻关课题(971200101-13)

摘　　要：目的探讨端粒酶活性检测在乳腺癌临床诊治中的意义。方法采用TRAP-assay法检测了人乳腺癌及癌旁正常组织中端粒酶活性。结果细胞株HeLa细胞、K562细胞端粒酶活性均为阳性表达,26例乳腺癌组织端粒酶活性均阳性表达(其中4例呈弱阳性),24例癌旁组织中5例呈弱阳性,19例呈阴性,而lysisbuffer及阴性对照均呈阴性反应。结论端粒酶在人乳腺癌的发生与发展中可能起着重要作用,提示端粒酶活性测定可用于乳腺癌的早期诊断。Objective To evaluate the role of telomerase activity in development of breast cancer.Methods The telomerase activity in normal tissues、breast cancer and tumor-adjacent tissues were determined using PCR-based TRAP assay.Results Telomerase activity was detectable in HeLa,K562 and breast cancer cells were positive,5 of 24 tumor-adjacent tissue samples were light positive and 19 samples were negative.Conclusion Telomerase may play an important roal in the development of breast cancer.Our results indicate that the detection of telomerase activity may be useful in the diagnosis of breast cancer.

关 键 词：表达研究 端粒酶活性检测 细胞端粒酶活性 组织端粒酶活性 癌旁正常组织 HeLa细胞 buffer 人乳腺癌 阳性表达 酶活性测定 临床诊治 K562 癌旁组织 阴性对照 早期诊断 弱阳性 细胞株 性反应 

分 类 号：R737.9[医药卫生—肿瘤]