细胞因子诱导的NK细胞过继免疫治疗恶性肿瘤的初步应用  

The Clinical Application of Adoptive Immunotherapy with Cytokine Induced Natural Killer Cells in the Patients with Malignant Tumo

作　　者：黄朝晖[1] 华东[1] 王丰[1] 李莉华[1] 刘志辉[1] 

机构地区：[1]苏州大学附属第四医院肿瘤研究所,江苏无锡214062

出　　处：《江南大学学报（自然科学版）》2005年第2期209-211,共3页Joural of Jiangnan University (Natural Science Edition)　

基　　金：无锡市卫生局"十五"重大科研项目(锡卫科教[2002]3号)资助课题

摘　　要：目的　采集不同类型恶性肿瘤患者外周血,扩增CINK细胞,并观察自体CINK细胞过继免疫治疗的疗效和安全性.方法　利用抗CD3 单抗、IL-2、PHA从9 例肿瘤患者PBMC中诱导扩增CINK细胞,2 周左右开始自体回输.结果　CINK细胞经培养后细胞总数和CD3- CD56+NK细胞均获大量增殖.肿瘤患者经自体CINK 细胞免疫治疗后, CD3、CD4 T 细胞、NK 细胞、CD4/CD8的比例均显著提高(P<0.01),睡眠、食欲等指标也有相应改善,治疗过程中未见副作用. 结论　自体CINK细胞过继免疫治疗可以显著提高患者免疫功能,改善患者生活质量,且副作用小.OBJECTIVE To expand cytokine-induced natural killer cells(CINK) from patients with different malignant tumor, and assess the safety and therapeutic effects of adoptive immunotherapy with CINK. METHODS CINK cells were induced from PBMCs of 9 tumor patients by in vitro incubation with IL-2、anti-CD3 monoclonal antibody and PHA, then were transfused back to those patients about 2 weeks later. RESULTS After culture, the absolute number of CINK cells and CD3^-CD56^+ NK cells got obvious expanding. After being transfused with auto-CINK cells, the CD3 T, CD4 T, NK cell and CD4/CD8 of those patients were increased significantly ( P <0.01); indexes of life guality such as sleeping、 appetite、physical strength also got ameliorated; no obvious side effects were observed. CONCLUSION Adoptive immunotherapy of auto-CINK cells can significantly enhance cellular immune functions, ameliorate the quality of life and have few side effect.

关 键 词：肿瘤 过继免疫治疗 自然杀伤细胞 

分 类 号：R733.71[医药卫生—肿瘤]