乳酸菌受体菌株的筛选、鉴定及特性研究  

The Selection,Identification and Characteristic Study on a Host Strain of Lactic Acid Bacteria

作　　者：王荫榆[1] 陈丽珊[2] 贾士芳[3] 任大明[4] 郭本恒[1] 

机构地区：[1]光明乳业股份有限公司技术中心 [2]复旦大学遗传所国家重点实验室上海200433 [3]中国科学院微生物研究所 [4]复旦大学遗传所国家重点实验室

出　　处：《微生物学通报》2005年第2期60-64,共5页Microbiology China

摘　　要：从乳酸菌保健品中分离筛选到一株能作为受体菌的乳酸菌菌株COCC101,经鉴定为粪肠球菌(Enterococcusfaecali)。抗药性实验显示这株粪肠球菌对多数药物敏感或中度敏感;粪肠球菌中没有质粒存在,转化效率和电场强度有对应的正相关,最高达到2×104转化子/μgDNA,并且能广泛接受不同来源的质粒;在Nisin诱导下可表达外源的绿色荧光蛋白(GFP)。这些结果显示COCC101菌株在乳酸菌基因工程研究中有望成为受体菌。As a host strain, Lactic acid bacteria COCC101 strain, isolated from a capsule, was identified as Enterococcus faecali. The drug-resistance experiment indicated that it is sensitive or in medium sensitive to most of drugs. There is no plasmid in this strain. The transformation efficiency is in direct proportion to the voltage. The highest efficiency is 2×104 transposons/μg DNA. The strain can accept plasmids derived from different origin, and it expressed heterogeneous Green Fluorescent Protein (GFP) under the Nisin induction. All of these results indicated that the COCC101 strain is hopeful to be a host strain.

关 键 词：受体菌 鉴定 粪肠球菌 抗药性 转化效率 

分 类 号：Q939.97[生物学—微生物学]