牵张力对体外培养兔鼻软骨细胞影响的实验研究  

Experimental Study of Distraction Force on the Proliferation of Rabbit Nasal Chondrocytes in Vitro

作　　者：吴汉江[1] 白明海[1] 

机构地区：[1]中南大学湘雅二医院口腔科,湖南长沙410011

出　　处：《口腔医学研究》2005年第2期135-138,共4页Journal of Oral Science Research

基　　金：湖南省科技厅基金资助项目(01JZY2017)

摘　　要：目的:探讨牵张力对体外培养的不同年龄兔鼻软骨细胞增殖活性的影响及牵张力大小与兔鼻软骨细胞增殖活性改变的量效关系.方法:将第4代体外培养的新生及6周龄新西兰白兔兔鼻软骨细胞置于细胞膜式牵张力施加装置上培养,流式细胞仪检测不同的牵张力(5 kPa、10 kPa)在0～12 h内对兔鼻软骨细胞增殖活性的影响.结果:0～10 h内5 kPa牵张力组软骨细胞增殖指数随着牵张力作用时间的延长不断上升,增殖指数峰值位于10 h处;0～8 h内10 kPa牵张力组软骨细胞增殖指数随着牵张力作用时间的延长不断上升,增殖指数峰值位于8 h处;5 kPa较10 kPa牵张力对体外培养兔鼻软骨细胞具有更大的促增殖作用;牵张力对体外培养的新生兔兔鼻软骨细胞具有更大的促增殖作用.结论:牵张力可促进体外培养的新生及六周龄新西兰白兔兔鼻软骨细胞增殖活性,提示我们鼻软骨牵张方法不仅适用于临床矫治新生儿唇腭裂伴发鼻畸形,而且有可能用于矫治1岁左右婴儿甚至更大年龄患儿的唇腭裂伴发鼻畸形.Objective: The purpose of this study was to investigate the effects of distraction force on the proliferation of rabbit nasal chondrocytes in vitro and the relation between force distraction and proliferation rate of rabbit nasal chondrocytes. Methods: The fourth-passage chondrocytes were harvested from the nose of neonatal and 6-week-old rabbits. A continuous static distraction force was applied on the cells in vitro using a cellular static distraction device, and the proliferation of nasal chondrocytes was examined using a flow cytometry. The experimental groups were divided into seven groups under continuous static distraction force (5 Kpa, 10 Kpa) from 0 to 12 hours. Results: The results showed that the proliferation of nasal chondrocytes was promoted as the application time of static distraction force was prolonged (0~10 hours in 5 Kpa groups and 0~8 hours in 10 Kpa groups). The maximal proliferation appeared, when the nasal chondrocytes were cultured under 5 Kpa for 10 hours ,and 8 hours under 10 Kpa ( P <0.05). The proliferation of cells in the 5 Kpa group was more obvious than that in the 10 Kpa group ( P <0.05). The proliferation of cells in the neonatal rabbit group was more obviously than that in the 6-week-old rabbit group ( P <0.05). Conclusions: The data prove that static distraction stimulates in chondrocytes can influence and regulate the growth of nasal chondrocytes. It might provide a new methord to treat nasal deformity of cleft lip for the neonatal or even older children with the application of nasal cartilage distraction.

关 键 词：牵张力 鼻软骨细胞 增殖活性 

分 类 号：R782.2[医药卫生—口腔医学]