聚乙烯亚胺包裹的磁性纳米颗粒用于基因载体的实验研究  

An experimental study of PEI coated magnetic iron oxide nanoparticles as gene vectors

作　　者：韦卫中[1] 吴华[2] 徐春芳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院核医学科,湖北武汉430030 [2]福建医科大学附属厦门第一医院核医学科,福建厦门361003

出　　处：《医学研究生学报》2005年第4期302-305,F003,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30171062)

摘　　要：目的:探讨聚乙烯亚胺(PEI)包裹的氧化铁磁性纳米颗粒中polyMAG1000用于体外基因转导的可行性。方法:用扫描电镜观测polyMAG1000的粒径;观察在不同的酸碱度下,将polyMAG1000与pEGFP N1质粒DNA按不同的比例(v∶w)混合后,观察polyMAG1000结合和保护DNA的能力,并进行体外转染实验。结果:polyMAG1000的直径约100nm,颗粒均匀,无论在酸性、中性和碱性条件下均能与质粒DNA稳定地结合;polyMAG1000能把pEGFP质粒DNA导入肿瘤细胞中,进而表达绿色荧光蛋白。当polyMAG1000与DNA比例为1∶1时转染效率最高;加与不加磁场转染效率比较,差异具有显著性意义。结论:PEI包裹的氧化铁磁性纳米颗粒可用于体外基因转导,把PEI等基因导入载体与磁性纳米颗粒结合而形成的新型基因导入载体具有良好的应用前景。Objective:To evaluate the feasibility of using polyethyleneimine(PEI) coated magnetic iron oxide nanoparticles(polyMAG-1000) as gene vectors. Methods:The surface characteristies of the nanoparticles were observed with scan electronical microscope.The ability of the nanoparticles to combine and protect DNA were investigated at different PH after the polyMAG-1000 and DNA were combined at different ratio.The nanoparticles were tested as a gene vectors through transfection models in vitro. Results: Under scan electronical microscope, the diameter of the nanoparticles was about 100 nm. The nanoparticles could bind and condense DNA under acidic ,neutral and alkaline pH conditions. The nanoparticles could transfer gene into cell and express green fluorescent protein(GFP).The efficiency of transfection was the highest when the ratio of the nanoparticles and DNA was 1 ∶ 1(v ∶ w).The difference was marked in the transfection efficiency when magnetic field was added or not. Conclusion: The magnetic iron oxide nanoparticles coated with PEI may be potentially used as gene vectors.

关 键 词：磁性纳米颗粒 聚乙烯亚胺 非病毒载体 基因治疗 

分 类 号：R782[医药卫生—口腔医学]