实验性癫痫大鼠脑电调制与侧脑室注射催产素的作用  

Modulation effects of intracerebroventricular injection of oxytocin on electroencephalogram of experimental seizures in rats

作　　者：胡胜娣[1] 胡祁生[1] 

机构地区：[1]武汉大学医学院生理系,湖北省武汉市430071

出　　处：《中国临床康复》2005年第13期56-57,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察催产素对大鼠癫痫的作用效应并探讨其可能的作用机制。方法:实验在武汉大学医学院生理学电生理实验室完成。采用青霉素诱发的大鼠癫痫模型,动物随机分为6组,每组6只,第1组为对照组,第2,3,4组分别侧脑室注射催产素1,10,100ng,第5组注射催产素受体拮抗剂犤d(CH2)5-OVT犦1μg,第6组注射犤d(CH2)5-OVT犦1μg+催产素100ng。同步观察大脑额叶皮质和海马CA3区脑电图痫样波的发放频率、幅度以及放电形式的变化。结果:催产素100ng可明显增加痫样放电的频率犤由(22.83±0.70)次/min升至(39.67±2.53)次/min,t=5.777,P<0.01犦和幅度犤由(428.50±40.88)μV升至(540.24±71.12)μV,t=3.168,P<0.05犦;催产素10ng可使痫样放电的幅度增加犤(538.90±55.95)μV,t=4.004,P<0.05犦;犤d(CH2)5-OVT犦可阻断催产素的易化效应。结论:侧脑室注射一定剂量的催产素,可通过其受体作用加重实验性癫痫大鼠痫样放电。AIM:To observe the effects of oxytocin(OT) on epileptic seizures in rats, and study the possible mechanism. METHODS:The experiment was finished in the electrophysiological laboratory,Department of Physiology,Medical College of Wuhan University.Seizure models were established by using penicillin(PNC).Thirty six epileptic rats were divided randomly into 6 groups:control group and five administration groups with 6 rats in each group.In the five administration groups,the rats were given 1,10,100 ng OT,1 μg OT receptor antagonist[d(CH2)5 OVT],1 μg[d(CH2)5 OVT] +100 ng OT,into lateral cerebroventricle respectively.The electrocorticogram(EcoG) and the electrohippocampalgram(EHG) of the seizure models were observed. RESULTS: The discharge frequency increased from(22.83±0.07) to(39.67±2.53) times per minute(t=5.777,P< 0.01) and the amplitude increased from (428.50±40.88) to(540.24±71.12) μV(t=3.168, P< 0.05) of epileptiform discharges after the injection of 100ng OT; the amplitude enhanced after the injection of 10ng OT[(38.90±55.95) μV,t=4.004, P< 0.05]; and the facilitating effect of OT was blocked by the pretreatment with OT receptor antagonist[d(CH2)5 OVT]. CONCLUSION:Intracerebroventricular injection of OT may aggravate epileptiform discharges of experimental seizures in rats through OT receptor.

关 键 词：癫痫 催产素 脑电描记术 受体 催产素/拮抗剂和抑制剂 

分 类 号：R742.1[医药卫生—神经病学与精神病学]