大鼠脑组织经反复高加速度暴露后bcl-2,bax,p53和白细胞介素1β转化酶的表达(英文)  

mRNA expression of bcl-2, bax, p53,and interleukin-1 beta hydroxylase in rat brain following repeated high positive acceleration exposure

作　　者：刘红巾[1] 蔡庆[1] 姜树强[1] 姜建东[1] 

机构地区：[1]解放军空军总医院全军航空医学研究中心,北京市100036

出　　处：《中国临床康复》2005年第13期213-215,共3页Chinese Journal of Clinical Rehabilitation

基　　金：全军医药卫生科研基金课题(98D033);空军后勤部科研基金课题(KH9919076)~~

摘　　要：背景:反复高正加速度(+Gz)暴露可引起脑损伤,但其病理机制尚不清楚。目的:了解反复高正加速度(+Gz)暴露后大鼠脑组织中bcl-2,bax,p53和白细胞介素1β转化酶基因的表达变化,探讨细胞凋亡在反复高+Gz暴露所致脑损伤中的病理作用。设计:以SD大鼠为研究对象的随机对照实验研究。单位:一所医院的航空医学研究中心。材料:选用体质量为180～220g健康雄性SD大鼠26只,随机分成对照组和+Gz暴露组,其中对照组4只,+Gz暴露组22只。干预:将大鼠固定于动物离心机的转臂上,头朝向离心机轴心,+Gz暴露组条件为G值+14Gz,增长率1.5G/s,峰值持续时间45s,共作用3次,两次中间间歇0.5h。对照组大鼠亦在动物离心机上经历类似实验步骤,所承受的G值为+1Gz。分别于暴露后0.5,6.0,24.0和48.0h断头取脑。用半定量反转录聚合酶链反应方法检测对照组和+Gz暴露后0.5,6.0,24.0和48.0h大鼠脑组织中bcl-2,bax,p53和白细胞介素1β转化酶mRNA表达水平,并用原位末端脱氧核苷酸转移酶标记法检测脑组织中凋亡细胞。主要观察指标:各组大鼠+Gz暴露后大鼠脑组织中bcl-2,bax,p53和白细胞介素1β转化酶mRNA表达水平。结果:+Gz重复暴露后6h,大鼠脑组织bcl-2mRNA表达水平(0.32±0.08)明显低于对照组(0.69±0.15),bax,p53和ICEmRNA表达水平犤(.55±0.09),(BACKGROUND: Brain injury can be induced by repeated high positive acceleration(?Gz) exposure,but the mechanism was still unclear. OBJECTIVE:To investigate the role of apoptosis in higher ?Gz exposures induced brain injury by observing the changes of mRNA expression of bcl 2,bax,p53,and interleukin 1β(IL 1β) hydroxylase in rat brain. DESIGN:Randomized control experimental study based on experimental SD rat model. SETTING:Aviation medicine research center of a hospital. MATERIALS:Twenty six healthy male SD rats,weighed from 180 to 220 g,were randomly divided into control group(4 rats) and ?Gz exposure group(22 rats). INTERVENTIONS:Rats were fixed on the rotating arm of animal centrifuger with their heads towards axis.Rats in ?Gz exposure group were exposed to?14 Gz for three times,each for 45 seconds with 30 minutes interval in between.Rats in control group were subject to the same experiment in?1 Gz.The rat brains were taken 30 minutes,6 hours,24 hours and 48 hours after the last centrifuge run, and then fixed and embedded.Changes of bcl 2,bax,p53,and IL 1βhydroxylasein mRNA expressions in rat brain were measured with semi quantitative reverse transcription polymerase chain reaction(RT PCR).Apoptotic cells were detected by terminal deoxynucleotide(correction of deoxynuleotide) transferase mediated dUTP nick end labeling(TUNEL) technique. MAIN OUTCOME MEASURES:The changes of bcl 2,bax,p53, and IL 1βhydroxyalse mRNA expressions at each time points. RESULTS:After repeated ?Gz exposures for 6 hours,bcl 2 mRNA expression in rat brain tissue(0.32±0.08)was found significantly lower than in control group(0.69±0.15),while mRNA expressions of bax,p53,and interleukin 1βconverting enzyme(ICE) 0.55±0.09,0.48±0.12,0.79±0.12 were significantly higher than in control group 0.33±0.09,0.31±0.05,0.51±0.09(P< 0.01).After 24 hours of exposure,mRNA expression of bcl 2 in rat brain tissue (0.28±0.05) was significantly lower than in control group,while the mRNA expressions of bax,p53,and ICE 0.61±0.

关 键 词：脑/代谢 加速度 蛋白质p53/生物合成 细胞凋亡 原癌基因蛋白质c-hcl-2/生物合成 原癌基因蛋白质类/生物合成 

分 类 号：R743[医药卫生—神经病学与精神病学]