胃扩张对大鼠脑、脊髓和肌间神经丛Fos蛋白和降钙素基因相关肽表达的影响  

Effects of G astric D istention on the Expression of Fos Protein and Calcitonin Gene-related Peptide in Brain, SpinalC ord and M yenteric N erve Plexus of Rats

作　　者：章菲菲[1] 莫剑忠[1] 陈晓宇[1] 彭延申[1] 萧树东[1] 

机构地区：[1]上海第二医科大学附属仁济医院上海市消化疾病研究所,200001

出　　处：《胃肠病学》2005年第2期70-74,共5页Chinese Journal of Gastroenterology

摘　　要：背景:功能性消化不良是一种常见疾病,越来越多的证据显示其发病与内脏神经敏感性增高有关。目的:通过测定大鼠伤害性胃扩张后脑、脊髓和肌间神经丛Fos蛋白和降钙素基因相关肽(CGRP)的表达,探索内脏刺激传入的途径和方式。方法:24只成年Sprague-Dawley雄性大鼠随机分为3组:实验组(12只)、手术对照组(6只)和空白对照组(6只)。实验组和手术对照组先植入胃内气囊。48h后,实验组接受反复的气囊扩张[80mmHg(1mmHg=0.133kPa)],2h后处死全部实验动物,立即取材。采用免疫组化法观察脑、脊髓和肌间神经丛Fos蛋白和CGRP的表达。结果:实验组杏仁核、延髓和胸髓Fos蛋白的表达较其他两组显著增强(P均<0.01),肌间神经丛的Fos蛋白表达3组间无显著差异。实验组延髓、胸髓CGRP表达较其他两组显著增强(P<0.05和P<0.01)。延髓和胸髓中Fos蛋白与CGRP的表达显著相关(rs分别为0.794和0.728,P均<0.01)。结论:胃扩张刺激可以兴奋皮层下中枢,CGRP在内脏刺激信号的传入过程中起重要作用。Functionaldyspepsia is a com m on disease. M ore evidences show thatitis associated with visceral hypersensitivity.A im s:To detectthe expression ofFos protein and calcitonin gene鄄related peptide (CGRP)in brain,spinal cord and m yenteric nerve plexus of rats after nocuity gastric dilatation, and to determ ine the pathway and m ode of transm ission ofafferentvisceralstim uli.M ethods:Twenty鄄fourm ale Sprague鄄Dawley rats were divided random ly into three groups:experim entalgroup (n=12),surgery controlgroup (n=6)and norm alcontrolgroup (n=6).A balloon was im planted into the rats’stom ach in experim entalgroup and surgery controlgroup. Forty鄄eighthours later, the rats in experim ental group received repeated balloon dilatation [80 m m H g (1 m m H g=0.133 kPa)] lasting for2 hours. Then allrats were sacrificed, and the brain, thoracic spinalcord and gastric antrum were isolated and dissected. The expression ofFos protein and CGRP were exam ined by im m unohistochem istry. R esults: In the experim ental group, the Fos protein expression in am ygdaloid com plex, m edulla oblongata and thoracic spinalcord was significantly increased as com pared with thatin othertwo controlgroups (P<0.01).Sim ilarly,the expression ofCGRP in m edulla oblongata and thoracic spinal cord was also significantly increased as com pared with thatin other two controlgroups (P<0.05 and P<0.01).H owever no difference was found in Fos protein expression in m yenteric nerve plexus ofthe three groups. Fos protein expression was significantly correlated with CGRP expression in m edulla oblongata and thoracic cord, respectively (rs 0.794 and 0.728, P<0.01).C onclusions: Gastric distention can stim ulate lim bic system ,and CGRP plays an im portantrole in the afferent pathway ofvisceralstim uli.

关 键 词：肌间神经丛 胃扩张 相关肽表达 降钙素基因相关肽(CGRP) Sprague-Dawley 脊髓 大鼠脑 内脏神经敏感性增高 功能性消化不良 Fos蛋白表达 CGRP表达 实验组 免疫组化法 对照组 常见疾病 雄性大鼠 内脏刺激 气囊扩张 实验动物 

分 类 号：R573[医药卫生—消化系统]