抗4-1BB单克隆抗体诱导肿瘤细胞致敏的小鼠腹股沟淋巴结T细胞在体外表达IFN-γ  

Expression of IFN-γ of Mice TDLN T Lymphocyte in vitro under the Induction of Anti 4-1BB mAb

作　　者：陈兴[1] 于继云[1] 程绍辉[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《中国比较医学杂志》2005年第2期67-70,i001,共5页Chinese Journal of Comparative Medicine

基　　金：国家 8 63计划资助项目 [2 0 0 1AA2 1713 1]

摘　　要：目的　研究肾癌细胞致敏的小鼠腹股沟淋巴结T细胞在体外经抗4 - 1BB(肿瘤坏死因子受体家族成员)单克隆抗体诱导刺激后IFN γ的表达水平。方法　以BCG和高聚生作为佐剂,用6 0 Co射线灭活的小鼠肾癌细胞Renca刺激小鼠腹股沟淋巴结,致敏后的淋巴结(肿瘤引流淋巴结tumor draininglymphnode,TDLN)T淋巴细胞在体外联合使用IL 2、抗CD3、抗CD2 8、抗4 1BB小鼠单克隆抗体激活,然后利用流式细胞术(FACS)检测细胞内IFN γ(干扰素Ⅱ型)的表达水平。结果　应用4 - 1BB单抗刺激的实验组有抗瘤活性的T细胞比例明显提高,其中T淋巴细胞分泌IFN γ的水平达到4 8.2 3% (P <0 .0 1)。结论　抗4 1BB单抗体外刺激肿瘤细胞致敏的小鼠腹股沟淋巴结T细胞后,T淋巴细胞内表达IFN γ水平提高。Objective To study the activations and expression of IFN-γ of T lymphocyte of the sensibilizated lymph node of mice by mice renal cancer cell in vitro under the induction of Anti-4-1BB mAb. MethodsThe mice renal cancer cell line Renca had been inactivated by 60Co ray,then used for stimulating inguinal lymph node of BALB/C mice with BCG and Highly Agglutinative Staphylococcin as adjuvant. The T lymphocyte from this tumor-draining lymph node (TDLN) was activated using IL-2/Anti-CD3/Anti-CD28/Anti-4-1BB mAb in vitro. IFN-γ of T lymphocyte was detected by FACS. ResultsThe quantities of the activated T lymphocyte expressing IFN-γ was amplified up to 48.23%(P<0.01). ConclusionThe anti-4-1BB mAb may be the most important factor in raising the expression level of IFN-γ of the T lymphocyte from TDLN.

关 键 词：腹股沟淋巴结 单克隆抗体 肿瘤细胞 T细胞 小鼠 致敏 体外表达 肿瘤坏死因子受体 ^60Co射线 肿瘤引流淋巴结 IFN-γ水平 肾癌细胞 tumor T淋巴细胞 干扰素Ⅱ型 流式细胞术 细胞内表达 家族成员 IL-2 联合使用 抗CD3 CD28 

分 类 号：R695.805[医药卫生—泌尿科学] R392-33[医药卫生—外科学]