土槿甲酸对鼠肉瘤180细胞增殖的抑制作用  

Pseudolaric acid A for inhibiting the proliferation of mouse sarcoma 180 cell line

作　　者：王冶[1] 赵维诚[2] 车光升[1] 董瑶[1] 刘黎明[1] 

机构地区：[1]辽宁省基础医学研究所,辽宁省沈阳市110101 [2]辽宁省肿瘤医院附属肿瘤研究所,辽宁省沈阳市110042

出　　处：《中国临床康复》2005年第14期134-135,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨土槿甲酸对鼠肉瘤180细胞生长的抑制效应。方法:实验在辽宁省肿瘤医院附属肿瘤研究所完成。用不同浓度的土槿甲酸加入体外培养的鼠肉瘤180细胞中,观察加药后细胞在生长过程中数量及其形态的变化;用四甲基偶氮唑盐比色法检测土槿甲酸的细胞毒作用;用Hoechst33342和碘化丙啶双荧光染色法检测细胞凋亡。结果:药物浓度为0,10,1,0.1μmol/L时,经土槿甲酸处理24h后,鼠肉瘤180细胞凋亡率分别为1.5%,19.8%,49.9%,30.7%;经土槿甲酸处理48h后,细胞凋亡率分别为1.3%,75.2%,59.6%,39.8%;经土槿甲酸处理72h后,细胞凋亡率分别为0.9%,69.8%,50.3%,41.4%。土槿甲酸明显抑制鼠肉瘤180细胞生长,以10μmol/L的抑制作用为最强。结论:土槿甲酸能有效抑制鼠肉瘤180细胞的增殖。细胞凋亡率与药物浓度及作用时间呈正相关。AIM:To study the inhibitory effects of pseudolaric acid A(PAA) on the growth o f mouse sarcoma 180(S180) cell line. METHODS:The experiment was conducted in the Research Institute of Oncology,Lia oning Cancer Hospital.PAA at different concentration was added into the medium i n which S180 cells were cultured in vitro.The changes of quantity and morphology of cells were observed under phase contrast microscope after adiministration.Cy totoxicity of PAA was detected through Methyl thiazolyl tetrazolium(MTT).The r ate of cell apoptosis was calculated through Hoechst33342 and double fluorecent staining propidine iodide. RESULTS:When PPA at 0,10,1 and 0.1 μmol/L,the rate of cell apoptosis was 1.5 %,19.8%,49.9%,30.7%at 24 hours after treatment,1.3%,75.2%,59.6%,39.8%at 48 hours after treatment,and 0.9%,69.8%,50.3%,41.4%at 72 hours after treatme nt.The growth of S180 cell line was obviously inhibited by PAA,and most obviousl y when its concentration was 10 μmol/L. CONCLUSION:PAA can effectively inhibit the proliferation of S180 cell line.The apoptotic cell rate is of positive relationship with the drug concentration and administration time.

关 键 词：土荆(槿)皮 甲酸类 肉瘤180 细胞凋亡 

分 类 号：R73[医药卫生—肿瘤]