基质金属蛋白酶-7与喉鳞癌浸润转移及预后评估的相关性(英文)  

Correlation of matrix metalloproteinases-7 with the invasion,metastasis,and prognostic evaluation in laryngeal squamous cancer

作　　者：孙亚男[1] 王小红[2] 邓焕珍[3] 李源[2] 刘小华[4] 金德均[1] 李鹏[2] 

机构地区：[1]哈尔滨医科大学附属第二医院耳鼻咽喉科,黑龙江省哈尔滨市150086 [2]中山大学附属第三医院耳鼻咽喉科,广东省广州市510630 [3]中山大学附属第三医院急诊科,广东省广州市510630 [4]丽水市中心医院耳鼻咽喉科,浙江省丽水市323000

出　　处：《中国临床康复》2005年第14期195-197,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广东科技计划项目资助(2003C30309)~~

摘　　要：背景:基质金属蛋白酶-7在肿瘤浸润转移中具有重要作用,并与多种肿瘤的预后有关。目的:通过检测喉鳞癌组织中基质金属蛋白酶-7的表达,探讨其在喉癌浸润转移中的作用及其与预后的关系。设计:以细胞为研究对象的开放性实验研究。单位:两所大学医院的耳鼻咽喉科和一所市级医院的耳鼻咽喉科。材料:实验于2002-04/2003-01在哈尔滨医科大学附属第二医院中心实验室完成,材料为喉鳞癌和癌旁非癌组织,基质金属蛋白酶-7多克隆抗体及免疫组化试剂盒。方法:采用免疫组化方法检测70例喉鳞癌和癌旁非癌组织蜡块标本中基质金属蛋白酶-7蛋白的表达,运用反转录-聚合酶链反应和West-ern-blot技术检测35例喉鳞癌和癌旁非癌组织冻存标本中基质金属蛋白酶-7的表达。主要结局观察:观察基质金属蛋白酶-7的mRNA和蛋白表达结果与喉鳞癌浸润、转移及预后的关系。结果:70例喉鳞癌组织中基质金属蛋白酶-7蛋白表达阳性率为77.1%(54/70),癌旁非癌组织为5.7%(4/70)(P<0.01)。35例冻存癌组织中基质金属蛋白酶-7mRNA的表达阳性率为74.3%(24/35),癌旁非癌组织为5.7%(2/35)(P<0.01)。基质金属蛋白酶-7蛋白和mRNA水平的表达在浸润深度为T3～T4组明显高于T1～T2组,并且与颈淋巴结转移呈明显的正相关(P<0.01)。Western-blot显示28ku(基质金属蛋?BACKGROUND:Matrix metalloproteinases 7(MMP 7) plays an important role in the invasion and metastasis of cancer and is related to prognosis of many carcinoma s. OBJECTIVE:To investigate the significance of MMP 7 expression in the invasion and metastasis of laryngeal cancer and explore the relationship between MMP 7 expression and the prognosis of laryngeal squamous cell cancer(LSCC). DEGIGN:An open experimental study based on the cells. SETTING:Two otolaryngology departments of two university hospitals and one oto laryngology department of a municipal hospital. MATERIALS:The experiment was conducted in the Center Laboratory of Second Affi liated Hospital of Harbin Medical University from April 2002 to January 2003.The materials were laryngeal cancer and corresponding neighboring noncancerous tiss ues,MMP 7 antibody and immunohistochemical kit. METHODS:The expression of MMP 7 protein in 70 samples of paraffin embedded L SCC and corresponding neighboring noncancerous tissues was detected with immunoh istochemical technique.Reverse transcription polymerase chain reaction(RT PCR) and Western blot technique were used to examine the expression of MMP 7 in 35 frozen specimens of LSCC and corresponding neighboring noncancerous tissues. MAIN OUTCOME MEASURES:The relationship of MMP 7 mRNA expression with the inva sion depth(T grade), neck nodal metastasis and prognosis of LSCC. RESULTS:Immunohistochemical staining of MMP 7 was observed in 77.1%(54/70) o f cancer tissues and in 5.7%(4/70) of neighboring noncancerous tissues(P< 0.01) .The mRNA expression of MMP 7 was detected in 74.3%(24/35) of cancer tissues a nd in 5.7%(2/35) of neighboring noncancerous tissues(P< 0.01).The expression of MMP 7 was higher in T3-T4 group than that in T1-T2 group(P< 0.01),and was po sitively correlated with nodal metastasis(P< 0.01).Western blot showed that both 28 ku (latent form of MMP 7) and 19 ku(active form of MMP 7) bands had close relationship with T grade and neck nodal metastasis.Kaplan Meier survival curve showed that the group with

关 键 词：喉肿瘤 基质金属蛋白酶 肿瘤转移 预后 

分 类 号：R73[医药卫生—肿瘤]