浆膜腔积液细胞中Calretinin、CEA、p53、c-erbB-2表达及其意义  

Expression of Calretinin,CEA,p53 and c-erbB-2 in Cells in Serous Effusions and its Significance

作　　者：卢善明[1] 邱前程[1] 黄杰雄[1] 林莹[1] 

机构地区：[1]汕头大学医学院第一附属医院病理科,515041

出　　处：《实用癌症杂志》2005年第1期94-95,98,共3页The Practical Journal of Cancer

摘　　要：目的　探讨浆膜腔积液腺癌细胞和间皮细胞中Calretinin、CEA、p5 3、c erbB 2的表达及其意义。方法　应用细胞块免疫细胞化学方法,检测45例浆膜腔积液腺癌细胞和间皮细胞中Calretinin、CEA、p5 3、c- erbB- 2的表达水平。结果　Calretinin、CEA、p5 3、c erbB 2在腺癌细胞中阳性表达率分别为4% (1/2 6)、5 4% (14 /2 6)、69% (18/2 6)和77% (2 0 /2 6) ,间皮细胞中分别为79 % (15 /19)、16% (3 /19)、5 % (1/19)和2 1% (4 /19)。该4种标志物在腺癌细胞中阳性表达率与在间皮细胞中阳性表达率比较均有非常显著性差异(P <0 .0 1)。结论　Calretinin、CEA、p5 3、c- erbB- 2抗体联合检测可辅助鉴别腺癌细胞和间皮细胞。Objective To investigate the expression of Calretinin,CEA,p53 and c-erbB-2 in the adenocarcinoma(AC) and reactive mesothelium(RM) cells in serous effusions and its significance.Methods The expression of Calretinin,CEA,p53 and c-erbB-2 was detected by immunocytochemistry(ICC) in AC cells and RM cells in 45 serous effusions.Results The positive rates of Calretinin,CEA,p53 and c-erbB-2 were 4%(1/26),54%(14/26),69%(18/26) and 77%(20/26) respectively for AC cells,79%(15/19),16%(3/19),5%(1/19) and 21%(4/19) respectively for RM cells,showing a significant differences between AC cells and RM cells(P<0.01).Conclusion Combined detection of Calretinin,CEA,p53 and c-erbB-2 is an accessory method for differentiating between AC and RM.

关 键 词：CALRETININ c-erbB-2 浆膜腔积液 CEA P53 其意义 表达及 免疫细胞化学方法 间皮细胞 腺癌细胞 阳性表达率 显著性差异 方法应用 联合检测 细胞块 标志物 

分 类 号：R730.43[医药卫生—肿瘤] R737.9[医药卫生—临床医学]