p53在喉鳞状细胞癌及癌旁组织中的表达与生物学行为的关系及意义  

Effect of expression of p53 in squamous cell carcinoma of larynx and mucosa adjacent to tumor on the biological behavior

作　　者：罗克强[1] 王直中[2] 王娜亚[1] 张小伯[1] 杨继生 

机构地区：[1]北京中日友好医院耳鼻咽喉科,北京100029 [2]北京协和医科大学协和医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2005年第9期405-408,共4页Journal of Clinical Otorhinolaryngology

摘　　要：目的:研究并回顾性分析喉鳞状细胞癌(LSCC)及癌旁组织中p53基因的表达及其与生物学行为的关系和意义。方法:用免疫组织化学SP法检测76例LSCC及癌旁组织中的p53表达。结果:LSCC组织中p53过表达47.4%(36/76),对应原位癌p53过表达为40.0%(4/10);癌旁非典型增生组织中p53过表达22.2%(8/36);癌旁正常组织中p53无过表达(0/56);p53在LSCC实质及相应原位癌中的表达呈现完全一致性。p53在癌旁非典型增生组织和对应癌实质组织中的过表达呈现高度显著正相关性(P<0.01);p53在癌组织中的过表达显著高于非典型增生组织(P<0.01);p53过表达和性别、年龄、临床分期、临床LSCC类型(LSCC位置)、肿瘤大小均无统计学意义,和病理分级(G1→G2+G3)有高度显著相关性(P<0.01)。p53在LSCC淋巴结转移组的过表达(68.8%)高于非转移组中的过表达(41.7%),但差异无统计学意义。LSCC病理分级(G1→G2+G3)和淋巴结转移有高度显著相关性(P<0.01)。结论:p53异常表达可出现于LSCC不同阶段及癌旁癌前病变组织中,并具有显著相关性,是LSCC发生、发展的重要因素及有意义的早期改变,并可反映喉癌组织细胞增殖活性和恶性程度及预后。Objective:To investigate the expression of p53 in squamous cell carcinoma of larynx (LSCC) and mucosa adjacent to LSCC and study the correlation between it and the biological behavior with retrospective analysis.Method:p53 protein overexpression was detected in 76 squamous cell carcinoma and the tissue beside the LSCC with SP method of Immuno-histochemical staining[IHC].Result:p53 overexpression was observed in 47.4% of LSCC,40.0% of carcinoma in situ ,22.2% of atypical hyperplasia(AtH) ,0% of normal mucosa .p53 overexpression in parenchyma of LSCC as well as in the corresponding carcinoma in situ is unaminous completely.There was a significant difference between p53 positive staining in AtH and in carcinoma of larynx as well as carcinoma in situ,but p53 overexpression in AtH adjacent to the laryngeal carcinoma is apparently correlated with that in the corresponding parenchyma of carcinoma of larynx(P<0.01).No correlation was found between p53 over expression and sex,age,TNM stage,location and T stage,but there was significant correlation between p53 over expression and differentiation grading(G_1→G_2+G_3).It was noted that of the rate p53 overexpression was 68.8% in lymphatic metastasis group,which was higher than the percentage(41.7%) foand in lymphatic nonmetastasis group (P>0.05).Conclusion:p53 overexpression are present in LSCC of different stages and AtH with significant correlation.p53 may play an important role in an early stage of malignant transformation of a subset of LSCC.Multiple-step carcinogenesis and malignancy as well as prognosis may be in association with p53-related tumorigenesis.

关 键 词：P53 喉肿瘤 癌 鳞状细胞 原位癌 非典型增生 生物学行为 

分 类 号：R739.65[医药卫生—肿瘤]