葛根素对AGEs诱导CAM新生血管生成影响的实验研究  

The experimental study of angiogenes effect of puerarin on the chick embryo chorioallantoic membrane induced by advanced glycation endproducts

作　　者：茅彩萍[1] 顾振纶[1] 曹莉[1] 蒙东才[2] 江国荣[2] 

机构地区：[1]苏州大学医学院药理学教研室 [2]苏州市中医院,江苏苏州215007

出　　处：《中国药理学通报》2005年第4期420-423,共4页Chinese Pharmacological Bulletin

基　　金：江苏省中医药管理局科研基金资助项目(NoH 028)

摘　　要：目的　探讨葛根素对糖基化终产物(AGEs)诱导的鸡胚绒毛尿囊膜(CAM)新生血管生成的影响。方法　制备AGEs复合物,建立CAM模型,随机分为空白组、AGEs组,不同剂量葛根素组和阳性药对照组,采用单位面积血管百分比法,应用OPTIMAS软件,检测葛根素对AGEs诱导的CAM血管生成的影响。结果　不同剂量的AGEs均可明显促进CAM新生血管的形成(P<0 01),而大、中剂量葛根素对此有明显的抑制作用(P<0 05)。结论　葛根素对AGEs诱导的CAM新生血管的生成具有一定的抑制作用。Aim To study the angiogenes effect of puerarin on the chick embryo chorioallantoic membrane(CAM) induced by advanced glycation endproducts(AGEs). Methods To establish a model of AGEs inducing angiogenesis in CAM, and then CAM were divided randomly into six groups:blank control, AGEs, AGEs+Puerarin(0.1,1.0,10 μg ) and AGEs+5-FU(1.0 μg). The inhibition effects of puerarin were antomatically evalnated for blood vessels percentage in certain area using OPTIMAS.Results Our data demonstrated that AGEs is an angiogenetic factor, Puerarin( 1.0, 10 μg ) can inhibit AGEs inducing angiogenesis in CAM Angiogenic effects.Conclusion The results suggest that puerarin hold the effects of inhibiting AGEs inducing angiogenesis in CAM.

关 键 词：葛根素 糖基化终产物 鸡胚绒毛尿囊膜 血管增生 

分 类 号：R-332[医药卫生] R282.71