11,12-EET对在体大鼠正常及再灌注心肌JNK1/JNK2表达的影响  

Effects of 11,12-EET on the expression of phophorylatedJNK1/JNK2 in ischemic and reperfusion rat heart

作　　者：郝刚[1] 王红霞[1] 芦玲巧[1] 曾翔俊[1] 张立克[1] 

机构地区：[1]首都医科大学病理生理学教研室,北京100054

出　　处：《中国药理学通报》2005年第4期424-426,共3页Chinese Pharmacological Bulletin

基　　金：北京市卫生局基础与临床合作资助项目(No03JL01)

摘　　要：目的　观察11, 12- 环氧二十碳三烯酸对再灌注心肌JNK1 /JNK2表达的影响,探讨其与心肌保护作用的关系。方法　制备大鼠缺血/再灌注心肌损伤模型,动态观察心功能的变化;实验后取心肌,用Western Blot法测定心肌JNK1 /JNK2的表达。实验分①正常组(Norm);②假手术组(Sham);③缺血再灌注组(I/R);④短阵缺血预处置组(SI+I/R);⑤11, 12 -EET预处置缺血/再灌注损伤组(EET+I/R)。结果　再灌注30min,I/R组+dp/dtmax、-dp/dtmax和LVDP均低于Sham组、SI+I/R组和EET+I/R组(P<0 .05);而I/R组大鼠心肌JNK1 /JNK2磷酸化表达高于Sham组、Norm组及EET+I/R组(P<0. 05 ),EET+I/R组与Sham组间;SI+I/R组与I/R组差异均无显著性(P>0 .05)。结论　11, 12- EET具有保护心功能的作用,这种保护作用可能是通过抑制JNK1 /JNK2的表达。Aim To investigate the expression of phosphorylated JNK1/JNK2 and the protection of 11,12-EET in ischemic and reperfusion rat heart.Method The expression of JNK1/JNK2 was detected with western blot method and the changing of heart function during ischemia/reperfusion process was observed in different groups. Results The cardiac function (+dp/dt_(max)%,-dp/dt_(max)% and LVDP)of reperfusion periods(30 min) apparently decreased in ischemia/reperfusion (I/R) group contrasted with Sham group, short ischemia(SI)+I/R group and EET+I/R group,and the expression of phosphorylated JNK1/JNK2 increased in I/R group contrasted with nromal group,Sham group and EET+I/R group.Conclusion The myocardial protection of 11,12-EET ( 6.24×10^(-8) mol·L^(-1)) is able to inhibit the expression of phosphorylated JNK1/JNK2.

关 键 词：11 12-环氧二十碳三烯酸 再灌注损伤 预处置 C-JUN N端激酶 

分 类 号：R-332[医药卫生] R322.11