半边旗提取物5F体外对猪脑微管蛋白聚合的影响  

Effect of 5 F isolated from Pteris Semipinnata L on tubulin polymerization in vitro

作　　者：刘义 吴育廉[2] 邓亦峰 梁念慈 

机构地区：[1]广东天然药物研究与开发重点实验,广东湛江524023 [2]湛江海洋大学现代生物化学实验中心,广东湛江524025

出　　处：《中国药理学通报》2005年第4期447-449,共3页Chinese Pharmacological Bulletin

基　　金：国家自然基金资助项目(No39870900)

摘　　要：目的　研究半边旗提取物5F对微管蛋白聚合的影响。方法　在离体条件下,将受试化合物加入到微管蛋白聚合解聚反应体系中,于37℃、350nm下检测反应体系吸光值的变化。结果　5F能抑制反应体系吸光值升高,提示它对微管蛋白聚合具有抑制作用并能抑制其解聚。结论　5F能抑制体外微管蛋白聚合,从而可能是影响细胞的细胞周期及诱导凋亡途径之一。Aim To study the effect of 5F on tubulin polymerization.Methed 5F was added into the tubulin polymerization-depolymerization system and the changes of Absorption values at 350 nm, 37 ℃ were recorded. Results 5F decreased the Absorption values of the reaction system.and markedly inhibited not only tubulin polymerization but also tubulin depolymerization. Conclusion 5F inhibits tubulin polymerization and depolymerization, and leads to cell cycle arrested, and possibly induces apoptosis.

关 键 词：半边旗 5F 微管蛋白 

分 类 号：R-332[医药卫生] R282.71