虎杖苷对缺血缺氧下平滑肌细胞蛋白激酶C的影响  

Effect of PD on the activity of protein kinase c of vascular smooth muscle cells

作　　者：王月刚[1] 金春华[2] 黄海潇[3] 赵清[2] 吴平生[1] 

机构地区：[1]南方医科大学南方医院心内科,广东广州510515 [2]南方医科大学基础部实验教学管理中心,广东广州510515 [3]军事医学科学院放射医学研究所,北京100850

出　　处：《中国药理学通报》2005年第4期461-464,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No39870936)

摘　　要：目的　观察虎杖苷对缺血缺氧作用下血管平滑肌细胞(VSMC)蛋白激酶C(PKC)活性的影响以探讨其抗休克作用的机制。方法　取大鼠胸主动脉培养VSMC,用Koyama方法复制VSMC缺血缺氧模型。用液体闪烁计数仪测定PKC的活性。结果　缺血缺氧状态下VSMC胞浆PKC的活性上升(vs正常组P<0 .05),但胞膜的PKC活性下降(vs正常组P<0 .05),经PD治疗后胞浆PKC的活性下降(vs缺血缺氧组、治疗对照组P<0. 05,vs正常组P>0 05 ),胞膜的PKC活性上升(vs缺血缺氧组、治疗对照组P<0 .05,vs正常组P>0 .05)。结论　PD对缺血缺氧作用下的VSMCPKC的活性有调节作用,这可能是PD抗休克作用的分子机制之一。Aim To study the influence of PD on the activity of PKC(protein kinase C) of VSMC during ischemia and hypoxia.Methods The hypoxia model was made by drawing off the oxygen of an enclosed acryl glass box and ischemia model was made by low sugar culture medium. Then the activity of PKC was measured by γ-scintillation counting instrument.Results It was found that the activity of PKC of cytoplasm in VSMC in ischemia and hypoxia situation increased (vs normal group P<0.05),but the activity of PKC of cell membrane decreased(vs normal group P<0.05).When VSMC was treated with PD, the activity of PKC of cytoplasm in VSMC decreased and that of cell membrane increased (vs ischemia and hypoxia group P<0.05, vs normal group P>0.05 ).Conclusion PD can reverse the PKC activities induced by ischemia and hypoxia. It may be one of the mechanisms of PD to have antishock effect.

关 键 词：缺血 缺氧 平滑肌细胞 虎杖苷 蛋白激酶C 

分 类 号：R-332[医药卫生] R282.71