激光诱变选育高产PUFAs真菌菌株的研究  

Study on Screening of Fungi Strain with Highly Content PUFAs by Laser Mutation Method

作　　者：张晓昱[1] 邓张双[1] 朱长虹[2] 吕艳娜[2] 王宏勋[1] 

机构地区：[1]华中科技大学生命科学与技术学院,中国湖北武汉430074 [2]华中科技大学激光技术国家重点实验室,中国湖北武汉430074

出　　处：《激光生物学报》2005年第2期129-133,共5页Acta Laser Biology Sinica

摘　　要：采用PIV400型Nd:YAG激光器,对少见报道的产PUFAs丝状真菌菌株MonoblepharispolymorphaCornu进行激光诱变育种。在激光照射剂量:波长532nm,功率50mW,时间10min下,孢子致死率为75%～80%。突变株中ω3系列PUFAs正突变率大于80%,ω3系列突变株FPAm59传代10代以上性能稳定,在土豆基质上摇瓶培养6d后,生物量达到8g L,产油率53.4%,GLA343.1mg L、AA1209.0mg L、EPA125.1mg L、DHA119.4mg L;分别比对照菌株提高了2.5倍、9.7倍、18.0倍、6.6倍、10.3倍、3.8倍。结果表明,激光诱变育种是获得PUFAs高产菌株尤其是生产ω3系列PUFAs菌株的有效方法。In order to obtain mutant fungi strain with highly content PUFAs. The fungi spore in the plate were radiated at wavelength 532 nm for 10 minutes using Nd:YAG laser,the lethality ratio of spores is approximately 75%～80%.The mutation rate of series ω-3 is above 80%.the mutation strain FPAm59 can retain its stability during more than 10 passages.After six days culture,the biomass of FPAm59 is up to 8 g/L and 2.5 times of the control strains.The lipid component of the biomass is 53.4% and (9.7) times of control strains.The quantity of the GLA,AA,EPA,and DHA in the strain lipid is 343.1mg/L, 1 209.0 mg/L,125.1mg/L,119.4mg/L and 18.0 times,6.6 times,10.3 times,and 3.8 times of control strains lipid.Therefore,laser mutation to fungi is an effective method to obtain the high quantity of PUFAs especially to the ω-3 series.

关 键 词：激光诱变 真菌 PUFAS 

分 类 号：Q937[生物学—微生物学]