地高辛标记杜氏利什曼原虫kDNA重组片段特异性分析及序列测定  

ANALYSIS OF THE SPECIFICITY OF A KDNA FRAGMENT OF LEISHMANIA DONOVANI AND ITS SEQUENCING

作　　者：杨文天[1] 胡孝素[1] 吕洪刚[1] 

机构地区：[1]华西医科大学寄生虫学研究室

出　　处：《中国寄生虫病防治杂志》1994年第2期94-97,共4页Chinese Journal of Parasitic Disease Control

基　　金：国家教委博士点学科专项研究基金;卫生部青年科学研究基金

摘　　要：用地高辛（ｄｉｇｏｘｉｇｅｎｉｎ－１１－ｄＵＴＰ）标记杜氏利什曼原虫ｋＤＮＡ重组片段作为探针，与不同种株利什曼原虫ｋＤＮＡ斑点杂交，获得杜氏利什曼原虫一种特异性ｋＤＮＡ片段，双脱氧核苷酸链终止法测定该片段长３２１ｂｐ，计算机检索及同源性分析比较再次表明该片段具有较高的种特异性。该片段可作为探针用于利什曼病病原体的检测，有助于虫株鉴定，也可以作为聚合酶链反应（ＰＣＲ）扩增的靶序列。Using the recombinant plasmid pLK1 labelled by digoxigenin-11-dUTP as aprobe,dot hybridization was undertaken with the kDNAs from different Leishmania spp.and iso-lates respectively. The results showed that the inserted kDNA fragment was of L.donovanispecies specificity.Sequencing of this fragment showed it was 321 bp in length. Analysis of its’homology by comparison with the other known DNA sequences revealed that it was of speciesspecificity too.This fragmant can serve as a useful probe for identification of L.donovani speciesor a target for PCR amplification.

关 键 词：地高辛 杜氏 利什曼原虫 kDNA 序列 

分 类 号：R382.22[医药卫生—医学寄生虫学]