玉米根系蛋白磷酸酶基因ZmPP2C的克隆及表达特性  

Cloning and Expression Characteristics of Protein Phosphatase Gene ZmPP2C of Zea mays Roots

作　　者：徐云峰[1] 李大鹏[1] 谷令坤[1] 胡晓丽[1] 宗晓娟[1] 李德全[1] 

机构地区：[1]山东农业大学生命科学学院山东省作物生物学重点实验室,泰安271018

出　　处：《植物生理与分子生物学学报》2005年第2期183-189,共7页Journal Of Plant Physiology and Molecular Biology

基　　金：国家重点基础研究专项经费(No.G1999011700);国家自然科学基金项目(Nos.39870526;30471052)资助~~

摘　　要：依据植物蛋白磷酸酶2C(proteinphosphatase2C,PP2C)基因的保守区设计简并引物,运用RT-PCR方法,从玉米根系中分离到一个长度为936bp的PP2C基因的cDNA克隆,命名为ZmPP2C。Southern杂交结果表明它在玉米基因组中是低拷贝的,且存在一个小的PP2C基因家族。Northern杂交结果表明,不同玉米组织之间ZmPP2C的表达差异明显;分别用CaCl2、MgCl2、PEG、EGTA和ABA处理根系24h,只有CaCl2处理增加表达量,说明Ca2+能诱导玉米ZmPP2C基因在转录水平上的表达,或被依赖于Ca2+的方式诱导。According to the conserved motifs of plantprotein phosphatase 2C gene, degenerate oligonucle-otides were designed. A full cDNA sequence of PP2Cgene from Zea mays L. roots was cloned by RT-PCR.It was named ZmPP2C and had 936 bp. Southern blotshowed that the ZmPP2C gene was a low copy in theZea mays genome, and there was a small PP2C genefamily (Fig.4). Northern blot showed that the expres-sion of ZmPP2C gene was significantly different amongZea mays tissues (Fig.5). Zea mays roots treated withCaCl2, MgCl2, PEG, EGTA, and ABA for 24 h, the ZmPP2C expression increased only by CaCl2 treatment(Fig.6). It showed the transcription of ZmPP2C genewas induced by Ca2+, or in a Ca2+-dependent manner.

关 键 词：玉米根系 蛋白磷酸酶2C CDNA 表达特性 

分 类 号：S513[农业科学—作物学]