皮肤混合瘤中bcl-2和增殖细胞核抗原的免疫组化研究  

Immunohistochemical Study on Bcl-2 and PCNA in Mixed Tumor of Skin

作　　者：尤德渊[1] 王艳东[1] 尤海燕[2] 

机构地区：[1]尔滨医科大学第二附属医院皮肤科,150086 [2]黑龙江省医院皮肤性病研究中心

出　　处：《中华皮肤科杂志》2005年第5期300-302,共3页Chinese Journal of Dermatology

摘　　要：目的探讨bcl-2和增殖细胞核抗原(PCNA)在皮肤混合瘤中的表达及意义。方法采用免疫组化S-P法及免疫组化双染技术对12例皮肤混合瘤皮损标本中bcl-2和PCNA的表达进行观察。结果12例患者的皮损标本均见bcl-2及PCNA阳性染色。肿瘤巢内,衬于囊腔和导管样结构内侧的肿瘤细胞示PCNA阳性,而上皮样肿瘤细胞示bcl-2阳性。bcl-2阳性的肿瘤细胞多呈PCNA阴性染色,而bcl-2阴性的肿瘤细胞多呈PCNA阳性染色。结论皮肤混合瘤中bcl-2、PCNA的异常表达似与此肿瘤的发生相关。Objective To investigate the expression of bcl-2 and PCNA in mixed tumor of skin. Methods Bcl-2 and PCNA expression in 12 cases of mixed tumor of skin were studied using immunohistochemical S-P method and double-staining. Results Positive reactions of bcl-2 and PCNA were observed in all cases. PCNA staining was positive in tumor cells lining cystic and duct-like structures in tumor nests, but bcl-2 staining was positive in epitheloid tumor cells. Most tumor cells with bcl-2 were PCNA negative and vice versa. Conclusion Aberrant expression of bcl-2 and PCNA in mixed tumor of skin may play a role in tumorigenesis.

关 键 词：BCL-2 皮肤混合瘤 免疫组化研究 增殖细胞核抗原(PCNA) 免疫组化S-P法 阳性染色 肿瘤细胞 异常表达 细胞多 表达及 上皮样 标本 皮损 阴性 

分 类 号：R739.5[医药卫生—肿瘤]