水稻品种抗瘟性表型与抗病基因同源序列相似性关系  

Relationship Between Phenotype of Blast Resistance and Similarity of Resistance Gene (Analog) in Rice Varieties

作　　者：刘二明[1] 肖一龙 易有金[1] 庄杰云[4] 郑康乐[4] 罗峰 

机构地区：[1]湖南农业大学生物安全科技学院 [2]湖南亚华种业股份有限公司 [3]湖南省攸县农业局,湖南株洲412300 [4]中国水稻研究所 [5]湖南省攸县农业局

出　　处：《中国水稻科学》2005年第3期209-216,共8页Chinese Journal of Rice Science

基　　金：2001-2003年中国农业科学院博士后流动站湖南亚华种业股份有限公司博士后工作站资助项目。

摘　　要：用6对RGA引物,即RGA1(XLRR for/XLRR rev)、RGA2(XLRR inv1/XLRR inv2)、RGA3(NLRR for/NL RR rev)、RGA4(NLRR inv1/NLRR inv2)、RGA5(Pto kin1/Pto kin2)和RGA6(Pto kin3/Pto kin4)对21个品种进行RGA PCR的DNA指纹分析。以相似系数0.72和田间叶瘟严重度阈值0.84分别聚类,21个水稻品种均可以分成5类。尽管类与类之间没有一一对应关系,但抗谱广、抗性稳定或具持久抗瘟性的品种,能较好地聚为一类,如湘资3150、IR156、株两优02、ZR02。在6对引物中,RGA1和RGA2来自于水稻抗白叶枯病基因Xa21含LRR结构,RGA3来自于烟草N基因含LRR结构,上述3对引物比较适宜用于水稻稻瘟病抗性基因遗传背景分析。Six pairs of primers, viz. RGA1(Resistance gene analog) (XLRR-for/ XLRR-rev), RGA2 (XLRR-inv1/XLRR-inv2), RGA3(NLRR-for/NLRR-rev), RGA4 (NLRR-inv1/NLRR-inv2), RGA5 (Pto-kin1/Pto-kin2) and RGA6 (Pto-kin3/Pto-kin4), were used to fingerprint 21 rice varieties based on RGA-PCR. Clustering analysis showed that the 21 varieties could be both classified into 5 groups at 0.72 similarity coefficient or using index of leaf blast severity at 0.84 level. Although there is no parallelism relationship between group and group in two different types of the clustering, the varieties, such as Xiangzi 3150, IR156, Zhuliangyou 02 and ZR02 with broad spectrum or durable resistance can be finely fallen into the same group. It was also suggested that 3 pairs among 6 primer pairs, viz. RGA1 and RGA2 designed from the LRR region of rice Xa21 gene and RGA3 from the LRR region of tobacco N gene, were more suitable to evaluate rice germplasms for their genetic responses to rice blast by RGA-PCR.

关 键 词：抗病基因同源序列 水稻品种 相似性关系 DNA指纹分析 表型 持久抗瘟性 株两优02 抗白叶枯病 稻瘟病抗性 相似系数 对应关系 XA21 背景分析 基因遗传 for 引物 RGA 严重度 N基因 结构 叶瘟 田间 抗谱 烟草 

分 类 号：S511[农业科学—作物学]