树鼩脑局部血栓性缺血的扩布性抑制机制探讨  

Explore of Mechanism of Spreading Depression After Focal Cerebral Thrombotic Ischemia in Tree Shrews

作　　者：李树清[1] 张颖[1] 杨丽君[1] 孟强[1] 李家立[1] 李凡[1] 

机构地区：[1]云南省昆明医学院基础医学院,邮政编码昆明650031

出　　处：《微循环学杂志》2005年第2期4-7,F002,共5页Chinese Journal of Microcirculation

基　　金：云南省自然科学基金项目 (2 0 0 1C0 0 46M)

摘　　要：目的:观察树鼩血栓性脑缺血时,对侧神经元血小板活化因子(PAF)受体活化和神经元线粒体呼吸功能改变,探讨皮层扩布性抑制的可能机制。方法:采用光化学法诱导脑血栓形成,分别观察脑缺血对侧神经元超微结构、脑细胞膜PAF受体、单胺氧化酶(MAO)活性、单胺类递质含量以及神经元线粒体呼吸功能。结果:树鼩脑缺血时对侧皮层淤血,神经元线粒体肿胀;脑缺血4h对侧神经元PAF受体亲和量降低,其变化以24 h最为显著(P<0.01);缺血区线粒体Ⅲ态呼吸速率、呼吸控制率(RCR)及氧化磷酸化效率(P/O)均明显降低;缺血对侧皮层的P/O降低(P<0.05),Ⅲ态呼吸速率明显抑制(P<0.01);伴随着对侧脑组织MAO活性的升高(P<0.01),对侧皮层的去甲肾上腺素(NA)和5-羟色胺(5-HT)含量降低而5-羟吲哚乙酸(5-HIAA)含量明显升高(P<0.01)。结论:树鼩脑缺血时对侧皮层神经元PAF受体的活化以及MAO活性的增强可能在扩布性抑制的发生中具有重要作用。Objective: To observe the affinities receptor of platelet activating factor (PAF) and change of mitochondrial respiratory in the contralateral region, and to explore the possibility mechanism of spreading depression (SD) after focal cerebral thrombotic ischemia. Method: The focal cerebral thrombotic ischemia was induced by photochemical reaction in tree shrews. The ultrastruc-ture of neuron, the changes of PAF affinities receptor, the respiratory control rate (RCR) and phosphorus-oxygen ratio(P/O) of mitochondrial respiratory, the monoamine oxidase(MAO) activities and the monoamine neurotransmit-ters were examined respectively. Results: The affinities receptor of PAF in contralateral region is decreased at 4 hours after cerebral ischemia, letter index at 24 hours decreased significantly (P< 0. 01). The state Ⅲ respiratory of neuronal mitochondria, RCR and P/O are depressed markedly ( P < 0. 01). MAO activities in contralateral region is ascendant(P<0.01)and nore-pinephrine(NA) and 5-hydroxytryptamine (5-HT) contents decreased, and 5-hydroxyindole-3-acetic acid (5-HIAA) content increased (P<0.01) after thrombotic cerebral ischemia. Conclusion: The changes of affinities receptor of PAF and MAO activiting could possesse an important action on inducing SD after cerebral thrombotic ischemia.

关 键 词：扩布性抑制 机制探讨 血栓性 脑局部 树鼩 脑血管病变 脑血管疾病 缺血性中风 脑梗塞患者 脑卒中 脑部病变 发病机制 干预措施 临床观察 发病率 死亡率 患病率 病理性 侧肢体 脑缺血 增长 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R269.561[医药卫生—临床医学]