双氢麦角碱对血管性痴呆小鼠额叶皮层和海马组织cAMP水平的影响  

Effects of dihydroergotoxine to changes of cAMP level in frontal cortex and hippocampus of mice with vascular dementia

作　　者：王伟斌[1] 吕佩源[1] 尹昱[1] 梁翠萍[1] 樊敬峰[1] 

机构地区：[1]河北省人民医院神经内科,河北石家庄050051

出　　处：《中国新药与临床杂志》2005年第5期347-350,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：河北省自然科学基金资助项目(301415)

摘　　要：目的:观测血管性痴呆小鼠额叶皮层和海马组织环磷酸腺苷(cAMP)水平及双氢麦角碱对其的影响,探讨血管性痴呆发病的分子生物学机制。方法:通过双侧颈总动脉线结、连续3次缺血再灌注,制作血管性痴呆动物模型,并设立假手术组、双氢麦角碱组;术后d29,30分别测试学习和记忆成绩;应用放射免疫法检测小鼠额叶皮层和海马组织cAMP水平。结果:与假手术组比较,模型组学习和记忆成绩均降低(P<0.05),且额叶皮层和海马组织cAMP水平也降低(P<0.05);而与模型组比较,双氢麦角碱组学习和记忆成绩均改善(P<0.05),且额叶皮层和海马组织cAMP水平也升高(P<0.05)。结论:额叶皮层和海马组织cAMP水平降低可能参与了血管性痴呆的分子生物学发病机制;双氢麦角碱可以升高其cAMP水平而改善临床症状。AIM: To observe the cAMP level in frontal cortex and hippocampus of mice with vascular dementia (VD) and the effect of dihydroergotoxine (DHE) on it, and explore the molecular pathogenesis of VD. METHODS: The mice were subjected to ischemia-reperfusion of bilateral common carotid arteries for three times by making a temporary tie to establish a model of VD and the changes of learning abilities and memory were tested on d 29, 30 after operation. Sham-operated mice were made as a control group. DHE was administrated to another group of mice, which were taken as treatment group. The cAMP level was evaluated by the radioimmunoassay. RESULTS: Compared with sham-operated group, the learning abilities and memory of model group was worse (P<(0.05)) and the cAMP level in frontal cortex and hippocampus was lower (P<0.05); compared with model group, the learning abilities and memory of DHE group was better (P<0.05) and the cAMP level in frontal cortex and hippocampus was higher (P<0.05). CONCLUSION: The lower cAMP level in frontal cortex and hippocampus might be involved in the molecular pathogenesis of VD; DHE could improve the cAMP level and then ameliorate the clinical symptoms.

关 键 词：痴呆 血管性 环AMP 放射免疫测定 二氢麦角毒 

分 类 号：R749.1[医药卫生—神经病学与精神病学]