血管内皮生长因子防止脊髓运动神经元凋亡的研究  

Effects of vascular endothelial growth factor on apoptosis in spainal cord motor neurons

作　　者：耿喜林[1] 刘京升[1] 

机构地区：[1]兰州大学第二医院急救中心创伤骨科,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2005年第1期16-19,共4页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的研究成年大鼠脊髓损伤后血管内皮生长因子对运动神经元的神经保护作用以及Caspase3mRNA表达与运动神经元凋亡的关系。方法用72只SD大鼠制作成脊髓损伤模型,随机分为对照组、应用VEGF组和VEGF拮抗组。伤后6、12、24h和3、7、14d取材。应用原位杂交、TUNEL技术研究运动神经元的凋亡。结果Caspase3mRNA在VEGF组脊髓组织中的表达下调,在VEGF拮抗组中表达增高。TUNEL阳性细胞计数显示脊髓损伤后VEGF拮抗组有多量神经元丢失,而VEGF组神经元丢失数量明显减少。结论VEGF可以防止成年大鼠脊髓损伤后运动神经元的凋亡,其作用可能是由Caspase3介导的。Objective To study the neuroprotection of Vascular endothelial growth factor (VEGF) on motor neurons after spinal cord injury in adult rats and the relationship between caspase-3 mRNA expression and apoptosis of motor neurons.Methods Spinal cord injury models were established in 72 SD rats.All rats were divided into control group, VEGF treated group and anti-VEGF treated group.The spinal cord of T13 was obtained at 6,12,24h and 3,7,14d after injury.In situ hybridization and terminal deoxynucleotidyl transferase mediated nick end labelling (TUNEL) staining were used to study the apoptosis of motor neurons.Results The expression of Caspase-3 mRNA in spinal cord was down-regulation in VEGF group and up-regulation in ANTI-VEGF group.TUNEL positive cells show that a lot of motor neurons were lost in anti-VEGF antibody treated animals, but only a few of the neurons were lost in animals that received VEGF.Conclusion These results suggest that VEGF prevented the apoptosis of spinal motor neurons in adult rats after spinal cord injury, and that effect is mediated by caspase-3.

关 键 词：血管内皮生长因子 运动神经元凋亡 Caspase-3 防止 VEGF TUNEL技术 神经元丢失 神经保护作用 脊髓损伤 mRNA表达 成年大鼠 损伤模型 SD大鼠 原位杂交 表达下调 脊髓组织 细胞计数 对照组 拮抗 24h 

分 类 号：R735.2[医药卫生—肿瘤] R651.2[医药卫生—临床医学]