不同产地黄芪中山柰素的含量分析及HPLC测定  

HPLC Method to Measure the Kaempferol in Radix Astragali and the Analyzed of Content in Different Plazes

作　　者：白雪梅[1] 田嘉铭[1] 王德宝[1] 李铮 

机构地区：[1]河北北方学院实验中心,075000 [2]河北省张家口市中心血站,075000

出　　处：《河北北方学院学报（医学版）》2005年第1期38-39,共2页Journal of Hebei North University:Medical Edition

摘　　要：目的:建立测定黄芪中黄酮类成分山柰素的HPLC方法,并对不同产地黄芪中山柰素的含量进行分析。方法:YWG -C18色谱柱(4.6mm×2 5 0mm ,10 μm) ,甲醇与0 .4 %磷酸水溶液(70 :30 )溶剂洗脱,检测波长375nm ,柱温30℃,流速1.0ml.min- 1,进样量2 0 μl。结果:山柰素的回归方程:Y =1730 .75 838X±1.32 79795 ,r =0 .9998。平均回收率99.6 % (n =5 )。结论:不同产地黄芪中山柰素含量相差很大,本方法专属性强,重现性好,可对黄芪药材的质量控制提供依据。Objective: To develop a HPLC method to measure the Kaempferol of flavonoids composition in Radix astragali and analyze the Kaemp ferol quantity contained in Radix astragali grown in different plazes.Methods: YWG-C18 column (4.6mm×250mm,10μm),use the solution of MeOH and 0.4% H_3PO_4 solvent(70:30) to separate,the measuring wavelength is 375nm,the temprature of column is 30℃, and the flowing speed is 1.0ml·min -1 , injected is 2μl. Results: The regression equation of Kaempferol: Y=1730.75838X±1.3279795,r=0.999 8.The average retrieving rates are 99.6%.Conclusion: There are different plazes of their Kaempferol quantity contained. This method is good at its specialization and good restoration. It can provide scientific reference to centrolling the quality of Radix astragali.

关 键 词：山柰素 HPLC测定 黄芪 含量分析 产地 HPLC方法 黄酮类成分 平均回收率 溶剂洗脱 检测波长 回归方程 质量控制 色谱柱 水溶液 进样量 专属性 重现性 

分 类 号：TS207.3[轻工技术与工程—食品科学] TS245.9[轻工技术与工程—食品科学与工程]