白灵菇不同栽培菌株的酯酶同工酶多态性分析  

Esterase Isozyme Zymogram Polymorphisms of Cultivated Strains of Pleurotus nebrodensis

作　　者：霍云凤[1] 王振河[1] 王斌[1] 

机构地区：[1]河南科技学院,河南新乡453003

出　　处：《安徽农业科学》2005年第4期614-614,645,共2页Journal of Anhui Agricultural Sciences

基　　金：河南省新乡市科技发展计划项目(04N010)。

摘　　要：采用聚丙烯酰胺凝胶电泳技术对12个白灵菇栽培菌株酯酶同工酶的酶谱多样性进行了研究,根据酶谱相似系数计算了菌株间的平均遗传距离。结果显示:12个菌株共检出50条酶带,9种酶带类型,表明白灵菇的酯酶同工酶酶谱分布呈多态性。采用不加权平均法对菌株间的遗传距离进行聚类分析,从树状图反映出12个菌株被分成2大类,类间和类内菌株遗传变异程度不同,但总体上12个供试菌株的遗传变异并不丰富。Esterase isozyme zymogram polymorphisms of 12 caltivated strains of Pleurotus nebrodensis were investigated using vertical slab polyacrylamide gel electrophoresis. There were 50 bands of esterase isozyme in the zymograms of 12 tested strains. 12 strains were divided into nine groups according to their zymogram phenotypes. This result revealed that esterase isozyme zymograms assumed polymorphisms.The genetic distances between and among the strains were analyzed with Unweighted Pair Group Method using Arithmetic Average(UPGMA).12 tested strains were divided into two large groups. Within and among groups, there were various degree of genetic diversity. The dendrogram of cluster analysis based on genetic distance can exactly show genetic relationship among the 12 tested strains of Pleurotus nebrodensis.

关 键 词：白灵菇 栽培菌株 酯酶同工酶 聚类分析 

分 类 号：S646[农业科学—蔬菜学]