儿童急性白血病端粒酶活性与p15基因表达关系的研究(英文)  

Relationship between telomerase activity and expression of p15INK4b gene in children with acute leukemia

作　　者：文飞球[1] 陈亦欣[1] 曹励之[1] 刘冯[1] 周克英[1] 陈霆[1] 

机构地区：[1]暨南大学医学院附属二院(深圳市人民医院)儿科,广东深圳518020

出　　处：《中国当代儿科杂志》2005年第2期112-114,共3页Chinese Journal of Contemporary Pediatrics

基　　金：SupportedbyMedicalResearchFoundationofGuangdongProvince(A2001616)andInitiativeScientificResearchFoundationofMinistryofEducationofP.R.Chinaforoverseareturnees.

摘　　要：目的　探讨端粒酶活性与pINK4b(p15)基因表达在儿童急性白血病(AL)发病中的关系。方法　采用改良端粒重复序列PCR扩增和逆转录聚合酶链反应检测27例儿童AL骨髓单个核细胞端粒酶活性和p15基因表达情况,并与9例正常骨髓单个核细胞端粒酶活性及p15基因表达进行比较。结果　初诊时,AL患儿骨髓细胞端粒酶活性(34. 5±37. 0)TPG较对照组(2. 4±2. 2 )TPG明显增高(P<0. 001 ); AL患儿p15基因表达水平(9. 8±16. 2)%, 明显低于对照组(45. 8±16. 9)% (P<0. 001 )。AL患儿骨髓细胞端粒酶活性与p15基因表达水平之间无相关关系(r= 0.01304, P> 0. 05)。结论　端粒酶活化和p15基因表达水平降低与急性髓性白血病发展有关,但两者可能通过不同的机制作用于白血病。ObjectiveTo study the relationship between telomerase activity and p15INK4b (p15) expression in the development of childhood acute leukemia (AL). Methods Telomerase activity and p15 expression in bone marrow (BM) of 27 cases of childhood AL were evaluated using a modified telomeric repeat amplification protocol assay and reverse-polymerase chain reaction respectively. For comparative analysis, BM from control donors (n = 9) were analyzed. Results At diagnosis, the telomerase activity detected in BM from AL children was significantly higher than that from control donors (34.5±37.0 TPG vs 2.4±2.2 TPG, P < 0.001). The p15 expression in BM of AL children was significantly lower than that of the controls (9.8±16.2% vs 45.8±16.9%, P < 0.001). No significant correlation was observed between p15 expression and telomerase activity (r = - 0.01304, P >0.05). Conclusions The telomerase up-regulation and lower expression of p15 gene may play an important role in the development of AL by different mechanisms.

关 键 词：白血病 端粒酶 P15 INK4B 基因表达 儿童 

分 类 号：R733.71[医药卫生—肿瘤]