差别PCR技术检测食管癌C-erbB-2原癌基因的研究  

Study on Differential Polymerase Chain Reaction of C-erbB-2 Oncogene Amplification in the Esophageal Carcinoma

作　　者：杨文杰[1] 岳秀兰[1] 秦文斌[1] 曹虹然[2] 白雪峰[2] 

机构地区：[1]包头医学院基础医学部生物化学与分子生物学教研室,内蒙古包头014010 [2]包头市第七医院病理科

出　　处：《包头医学院学报》2005年第2期103-105,共3页Journal of Baotou Medical College

基　　金：内蒙古自然科学基金;批准号 980204

摘　　要：目的:了解食管癌C-erbB-2原癌基因扩增情况及与临床病理的关系。方法:应用差别PCR技术检测41例食管癌C-erbB-2癌基因扩增。结果:食管腺癌C-erbB-2扩增率为20. 0%,食管鳞癌C-erbB-2扩增率为38. 9%,两者差异无统计学意义。食管鳞癌C-erbB-2原癌基因扩增与组织学分化程度、淋巴结转移、浸润程度差异均无统计学意义。结论:C-erbB-2癌基因扩增与食管癌发生有一定关系,但可能与食管鳞癌预后无关。Objective:To explore the amplication of C-erbB-2 oncogene in esophageal carcinoma and its associationwith clinical pathology. Methods:C-erbB-2 oncogene amplification was examined by differential polymerase chain reaction in 41 cases of esophageal carcinoma.Results:In 14 out of 36 (38.9%) patients with esophageal squamous cell carcinoma and in 1 out of 5 (20.0%)patients with esophageal adeoocarcinomas,the carcinoma was found to be amplificated.No significant difference was noticed between the two groups in amplification of C-erbB-2.There is also no significant difference between the amplicntion of C-erbB-2 of ESC and hisllogical differentiation,lymph node metastasis and invasion. Conclusion:These results suggest that there is a certain relationship between amplification of C-erbB-2 and the occurrence of esophageal carcinoma,but amplification of C-erbB-2 may have no relationsip with the prognosis of patients with esophageal squamous cell carcinoma.

关 键 词：食管癌 C-ERBB-2 差别PCR 癌基因扩增 

分 类 号：R735.1[医药卫生—肿瘤]