MTT法检测子宫颈癌体外药物敏感性及其临床意义  

Detection of Chemosensitivity of Cervical Carcinoma in Vitro by MTT Assay and its Clinical Significance

作　　者：程玺[1] 蔡树模[1] 李子庭[1] 王华英[1] 薛木全[1] 唐美琴[1] 臧荣余[1] 凌云华[1] 

机构地区：[1]复旦大学肿瘤医院,上海200032

出　　处：《肿瘤学杂志》2005年第2期83-85,共3页Journal of Chinese Oncology

摘　　要：[目的]探讨子宫颈癌体外药物敏感性的临床意义。[方法]采用MTT法检测了20种常用化疗药物在46例子宫颈癌组织中的药物敏感性,计算其平均抑制率,并与临床研究中单药的化疗疗效比较。[结果]单药平均抑制率>20%的药物有DDP,CBP,NVB,Oxa,Vp鄄16,VM鄄26,e鄄ADM,MMC,ADM,THP,CPT鄄11,HCPT,Topotecan,MTX,VDS,Taxol,5鄄Fu,BLM,Gemzar。与体外药敏试验结果比较,多数临床经验用药也是体外药物敏感度较高的药物。[结论]体外药敏试验结果与临床化疗疗效并不完全一致,但体外药敏试验结果对于筛选和指导临床用药,提高化疗疗效有重要的临床意义。To investigate the clinical significance of chemosensitivity of cervical carcinoma in vitro. The chemosensitivity in vitro with 20 anti-cancer agents in 46 cases with cervical carcinoma was detected by MTT assay. The average of inhibitory rates was calculated, and compared with the clinical effects with single agent in clinic. The average of inhibitory rate higher than 20% in single drug was cisplatin, carboplatin, NVB, oxaliplation, etoposide, vumon, epirubicin, mitomycin-c, adriamycin, pirarubicin, irinotecan, hydroxycamptothecine, topotecan, methotrexate, vindersin, taxol, 5-fluorouracil, bleomycin, gemcitabin. Compared with the chemosensitivity in vitro, most of the anti-cancer agents used in clinic were the drugs with high chemosensitivity in vitro. [Conclusion] The results of chemosensitivity in vivo. However, it is useful for agents screening, guiding chemotherapy and improving clinical effects.

关 键 词：MTT法 宫颈肿瘤 药物筛选试验 抗肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]