基因芯片检测血清标本的丙型肝炎病毒基因型  

The genotypes of hepatitis C virus detected by HCV DNA microarray

作　　者：施英娟[1] 张冬雷[1] 王跃国[1] 张芹[1] 陈莲英[1] 毛红菊[2] 赵建龙[2] 毛丽萍[3] 王惠民[1] 

机构地区：[1]南通大学附属医院检验科,南通226001 [2]中国科学院上海微系统与信息技术研究所 [3]南通大学附属南通第三医院

出　　处：《南通大学学报（医学版）》2005年第2期92-93,96,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的:探讨丙型肝炎病毒(HCV )基因分型检测芯片在临床的初步应用及意义。方法:基因芯片检测15 0例血清标本中HCV基因型,并与HCV RNA定性及测序结果进行比较。结果:HCV基因分型检测芯片检出的阳性结果与HCV- RNA定性及测序结果的相对符合率为10 0 .0 %。结论:HCV基因分型检测芯片是一种准确性好、灵敏度高、特异性强、操作简便快速的基因检测方法,可以为临床诊断。Objective:To approach the primary application and significance of HCV genotyping chip in the clinical practice.Methods:The results from 150 serum samples detected by HCV genoyping chip were compared with those from traditional RT-PCR and sequencing.Results:There was 100.0% coincidence between HCV genotyping chip and the others.Conclusions:HCV genotyping chip is a fast and easy method for genotyping which shows higher specificity and sensitivity,so it can play an important part in the diagnosis and therapy.

关 键 词：基因芯片检测 丙型肝炎病毒基因型 血清标本 丙型肝炎病毒(HCV) HCV基因分型 检测芯片 HCV基因型 HCVRNA 基因检测方法 初步应用 阳性结果 临床诊断 符合率 准确性 灵敏度 特异性 测序 定性 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R512.63[医药卫生—临床医学]