原发性肝细胞癌中p53、p16和p21^(WAF1)蛋白表达及临床意义  

Clinicopathological significance of the expression of p53, p16 and p21^(WAF1) proteins in primary hepatocellular carcinoma

作　　者：顾春燕[1] 姚建国[2] 刘宏斌[1] 包蕾[1] 

机构地区：[1]南通大学附属第三医院病理科,南通226006 [2]南通大学第二附属医院病理科

出　　处：《南通大学学报（医学版）》2005年第2期94-96,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的:研究p5 3、p16和p2 1WAF 1蛋白在原发性肝细胞癌中的表达及临床意义。方法:应用免疫组织化学S- P法检测原发性肝细胞癌4 1例,癌旁组织30例中p5 3、p16和p2 1WAF1 蛋白的表达。结果:p5 3蛋白的表达在PHC中显著高于癌旁(P<0 .0 1) ,其表达与病理学分级、有无肝内或门静脉转移有显著相关性(P<0 .0 5 )。p16蛋白的表达在PHC中显著低于癌旁(P<0 .0 1) ,其表达与病理学分级显著相关(P<0 .0 5 )。p2 1WAF 1 蛋白的表达在PHC中显著高于癌旁(P<0 .0 1) ,而在不同临床病理特征间差异无显著性。结论:p5 3、p16和p2 1WAF 1蛋白的表达情况与原发性肝细胞癌的发生、发展及恶性程度有关。Objective:To investigate the expression and significance of p53, p16 and p21 WAF1 proteins in primary hepatocellular carcinoma(PHC). Methods:Immunohistochemical (S-P) method was used to detect the expression of p53,p16 and p21 WAF1 proteins in PHC tissues from 41 patients and para-cancer tissues from 30 patients.Results:The expression of p53 protein was significantly higher in tumor than that in corresponding paracancerous tissue(P<0 01).There was notable relationship between the expression of p53 and differentiating grades,intrahepatic metastasis and thrombus in the portal veins(P<0.05).The expression of p16 protein was considerably lower in tumor than that in adjacent tumor tissue(P<0.01),and was associated only with differentiating grades(P<0.05) .The expression of p21 WAF1 protein was also higher in tumor(P<0.01),but not related to any clinicopathological characters.Conclusion:Expression of p53, p16 and p21 WAF1 proteins is closely correlated to the occurrence, development and malignancy degree of hepatocellular carcinoma.

关 键 词：P21^WAF1蛋白 原发性肝细胞癌 临床意义 P16 表达及 病理学分级 免疫组织化学 临床病理特征 p53蛋白 P16蛋白 PHC 癌旁组织 静脉转移 表达情况 恶性程度 相关性 显著性 

分 类 号：R734.2[医药卫生—肿瘤] R735.7[医药卫生—临床医学]