放射性核素内照射诱发大鼠脾淋巴细胞的HPRT基因突变  

Study on HPRT Gene Mutation of Rat Spleen Lymphocytes Induced by Internal Exposure to Radio-nuclides Using Cloning Method

作　　者：崔凤梅[1] 赵经涌[1] 劳勤华[1] 王六一[1] 宁萍[1] 洪承皎[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,江苏苏州215007

出　　处：《工业卫生与职业病》2005年第3期140-143,共4页Industrial Health and Occupational Diseases

基　　金：国防科工委基金资助项目(Y5573062)

摘　　要：目的　探讨克隆法研究放射性核素内照射诱发大鼠脾淋巴细胞HPRT基因突变的可行性。方法　大鼠尾静脉注入晚期混合裂变产物, 克隆法检测不同累积剂量和不同剂量率的内照射诱发的脾淋巴细胞HPRT基因突变, 拟合剂量-效应方程。结果　随着累积剂量和剂量率的增加, HPRT基因突变频率随之上升, 剂量-效应关系和剂量率-效应关系均符合线性平方模型, 分别为: y=4 506 0+0 563 5D+0 060 6D2, y=2 663 8+0 517 7D-0 000 8D2。结论　克隆法是一种敏感的检测辐射诱发HPRT基因突变的方法, 脾淋巴细胞的HPRT基因突变对辐射敏感。Objective To study HPRT gene mutation of rat spleen lymphocytes induced by internal exposure to radio-nuclides using cloning method.Methods The radio-nuclides were injected into the rats via tail vein and the HPRT gene mutation was detected by cloning method to study the relationship of dose vs.response and of dose-rate vs. response.Results The HPRT gene mutation frequency rose with the incresae of accumulative dose and the dose-rate,both dose-response and dose-rate response relationships could be described as linear-quadratic models.Conclusions Cloning method is a sensitive method to detect HPRT gene mutation induced by radiation,and the HPRT gene mutation of spleen lymphocytes is sensitive to radiation.

关 键 词：克隆法 放射性核素 内照射 基因突变 剂量-效应关系 

分 类 号：R818.03[医药卫生—放射医学]