HBsAg阳性孕妇细胞转运意义的研究  

Study on HBsAg positive pregnant women cell transportion

作　　者：魏俊妮[1] 王素萍[1] 冯丽萍[2] 双杰玉[2] 

机构地区：[1]山西医科大学流行病学教研室,太原030001 [2]太原市传染病医院妇产科

出　　处：《中国公共卫生》2005年第5期536-538,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目 (30 0 70 669)

摘　　要：目的　了解HBsAg阳性孕妇细胞是否可进入胎儿血循环,探索母儿细胞转运与乙型肝炎病毒(HBV)宫内感染关系。方法　用等位基因特异性PCR(allele specficPCR ,AS PCR)和半巢式PCR(hemi nPCR)技术扩增新生儿外周血中母亲-S -DNA ,判定母儿细胞转运。非条件Logistic回归模型分析母 儿细胞转运与HBV宫内感染关系。结果　以谷胱甘肽转移酶(GST)M 1、血管紧张素转换酶(ACE)基因作为母亲的特异性等位基因,检测4 2对信息病例对中2 6例(6 1. 90 % ,2 6 /42 )新生儿发生了母 儿细胞转运。母 儿细胞转运、孕妇外周血单个核细胞(PBMC)HBVDNA阳性是HBV宫内感染的危险因素,OR值分别为8 .4 0 (1 .4 2～4 9. 74 ) ,8. 78(1 .2 3～6 2 . 6 9)。结论　母 儿细胞转运可能是HBV宫内感染的重要原因,PBMC可能介导HBV穿过胎盘屏障感染胎儿。Objective To study the route that the HBsAg positive preg nant women's cell entered fetal circulation,and to probe into the association be tween the cell transportion from mother to baby and HBV intrauterine infection.Methods The cell transportion from mother to baby were de termined by allele-specific PCR(AS-PCR) and heminested PCR(hemi-nPCR).The ass ociation between the cell transportion from mother to baby and HBV intratuerine infection was analyzed by noncondition Logistic regression model.Results GSTM1,ACE as maternal specific alleles:42 mother -baby pairs were recruited from hemocyte specimens as informative cases,26(61. 09%) newhorns' blood cell out of 42 informative cases had maternal cell DNA.The cell transportion from mother to baby and PBMC HBV infection in pregnant women w ere risk factors of HBV intrauterine infection,OR were respectively 8.40(1 .42-49.74)、(OR=8.78,1.23-62.69).Conclusions The cell transportation from HBsAg positive m others to baby had more contribution to HBV intrauterine infection;HBV infection fetus was probably mediated by PBMC to pass through the placentae barrier and i nfect fetus.

关 键 词：宫内感染 等位基因 PBMC HBV PCR 

分 类 号：R512.62[医药卫生—内科学]