5种习用柴胡的ITS序列鉴别  

ITS sequence identification of Radix Bupleuri

作　　者：武莹[1] 刘春生[1] 刘玉法[1] 阎玉凝[1] 

机构地区：[1]北京中医药大学中药学院

出　　处：《中国中药杂志》2005年第10期732-734,共3页China Journal of Chinese Materia Medica

摘　　要：目的:用PCR扩增方法测定5种常用柴胡的ITS序列,为柴胡的分子鉴定提供依据。方法:分别从5种柴胡的叶片中提取总DNA,以核基因组通用引物进行扩增,扩增产物经纯化后,用PCR产物直接测序。结果:各样品的ITS1长度为214～220bp,ITS2长度为230～231bp。5种柴胡的ITS序列分别有多个特异性信息位点。结论:ITS序列可以作为柴胡分子鉴定的依据。Objective: To provide the basis of molecular authentication of Radix Bupleuri by the comparison of the internal transcribed spacer(ITS) sequences of five kinds of Radix Bupleuri in common use. Method: Firstly, total DNA of five kinds of Radix Bupleuri was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR product was directly sequenced after purification. Result: The length of ITS1 and ITS2 sequence was 214～220 bp and 230～231 bp respectively. There were distinct variation sites between the ITS sequences of the five kinds of Radix Bupleuri. Conclusion: ITS sequence may be the evidence for the molecular authentication of Radix Bupleuri.

关 键 词：ITS序列 柴胡 鉴别 分子鉴定 PCR产物 扩增方法 总DNA 通用引物 扩增产物 直接测序 ITS1 ITS2 基因组 性信息 依据 长度 

分 类 号：S567.79[农业科学—中草药栽培]