大肠癌DNA含量与其生物学行为关系探讨  

THE RELATIONSHIP OF DNA CONTENT TO BLOLOGICALBEHAVIOR IN HUMAN COLORECTAL CARCINOMA

作　　者：温增庆[1] 黄顺生[1] 姚育修[1] 彭立民[1] 

机构地区：[1]江西医学院第一附属医院普外科

出　　处：《中国普通外科杂志》1994年第2期72-74,共3页China Journal of General Surgery

摘　　要：作者应用流式细胞仪（ＦｌｏｗＣｙｔｏｍｅｔｒｙ，ＦＣＭ）分析测定４４例手术切除大肠癌标本，旨在探讨大肠癌的生物学特性与临床的关系。结果表明：①非整倍体大肠癌，其生物学行为更具恶性特征：癌生长＞１／３圈肠腔的肿瘤，非整倍体检出率高达８０％；②术后生存期少于２年的患者其检出率为７３％，明显高于生存期３年以上用者检出率３０％（Ｐ＜０．０５）；③大肠癌细胞增生指数均值０．３７±０．０８，高于正常大肠粘膜均值０．３０±０．０５（Ｐ＜０．０５）。高增生指数大肠癌易于腹膜种植转移。作者认为ＦＣＭ能客观反映大肠癌生物学特征，术前行ＦＣＭ测定，可为大肠癌的治疗方案设计及估计预后提供重要依据。he nuclear DNA content of 44 human colorectal turnors was measured bymeans of flow cytometry, and compared with 10 cases with normal colorectalmucosa. The results showed that aneuploid tumor had more invasive biologiccharacterstics and greater tendency to metastasis. The DNA content was signifi-cantly related tothe extent of tumor growth along the colon wall. 55 percent ofdiploid grow less than 113 circumference of the wall. It suggests that tumorwhich grows to 113 circumference of the colon wall may be a critical point atwhichthe biological characteristics of colorectal cancer would change. Patientswho survived less thanZ years have more aneuploid patterns than the patientswho survived beyond 3 years (P<0. 05). FCM can provide with an objective andquantitive reference. It is useful in diagnosis and prognosis of the tumor.

关 键 词：流式细胞仪 DNA 大肠肿瘤 生物学 

分 类 号：R735.340.2[医药卫生—肿瘤]