甘蔗杂种的染色体和RAPD鉴定研究  

Identification of Sugarcane Hybrids Based on Chromosome Number and RAPD

作　　者：李富生[1] 林位夫[1] 何顺长[2] 

机构地区：[1]中国热带农业科学院橡胶栽培研究所,海南儋州571737 [2]云南农业大学甘蔗研究所,昆明650201

出　　处：《植物遗传资源学报》2005年第1期48-52,共5页Journal of Plant Genetic Resources

基　　金：云南省自然科学基金项目(2001C0015Q)资助

摘　　要：采用体细胞染色体计数和RAPD(Random Amplified Polymorphism DNA)分子标记的方法鉴定甘蔗与蔗茅属间杂交种F1的真实性。结果表明,杂种F1材料01/47、01/85、01/120的2n=80-82,染色体遗传方式为n+2n。用110个随机引物进行RAPD扩增,63个引物可获得双亲的RAPD多态性,其中3个引物OPC-19、OPE-2、OPF-4可在杂种F1材料01/64和01/120的RAPD扩增标记中显示出双亲的特征谱带,分别为父本3500bp和母本1300bp、父本1350bp和母本900bp、父本550bp和母本900bp。Some intergeneric hybrids (Sugarcane × Erianthus fulvus ) were identified by chromosome counting and RAPD (Random Amplified Polymorphism DNA) technique. The research results revealed that hybrids 01/47, 01/85 and 01/120 had 2n = 80 - 82 chromosome number. The chromosome transmission of their parents was a type of 'n + 2n'. Of 110 RAPD primers used, 63 were polymorphic. The three primers, OPC-19, OPE-2 and OPF-4 were found to be able to determine hybrids 01/64 and 01/120, which possessed maternal-specific and paternal-specific DNA bands, 1300bp and 3500bp, 900bp and 1350bp, 900bp and 550bp, respectively.

关 键 词：RAPD鉴定 甘蔗 RAPD扩增 RAPD多态性 杂种F1 染色体计数 属间杂交种 分子标记 遗传方式 随机引物 特征谱带 体细胞 真实性 母本 父本 材料 双亲 蔗茅 

分 类 号：S566.1[农业科学—作物学]