生山栀-冬虫夏草复方不同方法提取物对急性呼吸窘迫综合征的作用  

Effects of Gardenia-Aweto compound by different extraction method on antagonizing acute respiratory distress syndrome

作　　者：严冬[1] 李兰娟[1] 杜维波[1] 任国平[1] 曹红翠[1] 朱狄峰[2] 夏蕾[2] 曾真[2] 邵青[2] 张海江[2] 程翼宇[2] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003 [2]浙江大学药学院中药科学与工程学系,浙江杭州310027

出　　处：《中国中药杂志》2005年第9期700-703,共4页China Journal of Chinese Materia Medica

基　　金：浙江省科技厅重大科研项目(G20030548)

摘　　要：目的:观察不同提取方法的生山栀冬虫夏草复方提取物对兔急性呼吸窘迫综合征(ARDS)的治疗作用。方法:采用水提及醇提的方法,获水提取液(总提液)、复方醇溶提取液(醇溶液)、复方醇沉(醇沉液)提取液。静脉注射油酸复制兔ARDS模型,观察该复方不同方法提取物对兔急性呼吸窘迫综合征血气分析、肺系数、肺泡灌洗液(BALF)白蛋白含量及肺部病理改变的影响。结果:总提液、醇溶液能提高兔ARDS的血氧饱和度,降低肺系数、肺泡灌洗液白蛋白含量,减轻肺损伤程度。醇沉液则对上述指标改善作用不明显。结论:预防性应用总提液、醇溶液能有效改善油酸型兔ARDS肺功能。Objective: To study the effect of Gardenia-Aweto compound (GAC) and two component on preventing acute respiratory distress syndrome (ARDS) by the rabbit model of ARDS induced by intravenous injection of oleic acid. To detect the efficiency component of GAC in preventing ARDS. Method:GAC was divided into two compounts, ethanol-soluble components (ESC) and ethanol-deposition components (EDC),based on polarity. Forty-three new zealand rabbits were randonlly divided into five groups, the blank control group, the model group, the GAC groups, the ESC group, and the EDC group. The ARDS model was induced by intravenous injection of oleic acid. Dynamic changes of arterial blood gas, lung index, albumin in bronchoalveolar lavage fluid(BALF) in different groups and lung histological changes were observed and compared. Result:As compared with the blank group, in the model group, GAC group, ESC group, EDC group the arterial PO 2 and oxygen saturation deprived continuously. While SO 2 in GAC group at time points 30,60,90,120 min (P<0.05 or 0.01) and SO 2 in ESC group at time points 30,60,90 min were higher than those in ARDS group. PO 2 in ESC group at time points 30,60 min(P<0.05) were higher than those in ARDS group. The value of LI and W/D were higher in ARDS group than in sham group (P<0.01),they were much lower in HD group than in ARDS group (P<0.01). Concentration of BALF-albumin increased markedly in ARDS group and pre-treatment groups compared with sham group, but it was much lower in GAC group and ESC group, there was a significant difference between GAC group (P<0.01), ESC group (P<0.05) and ARDS group. The lung histological changes had been improved in GAC group and ESC group. But no significantly difference between above-mentioned parameters was found in comparison in the model group and in the EDC group. Conclusion:Preventive administration of GAC or ESC an protect the damaged lung function in ARDS rabbits induced by oleic acid. The efficiency component of GAC in preventing ARDS is ESC. GAC antagonizing ARDS may r

关 键 词：冬虫夏草 急性呼吸窘迫综合征(ARDS) 山栀 肺泡灌洗液 不同提取方法 静脉注射油酸 蛋白含量 复方提取物 血氧饱和度 预防性应用 醇溶液 治疗作用 水提取液 血气分析 病理改变 损伤程度 改善作用 肺系数 提液 醇沉 肺功能 

分 类 号：R285[医药卫生—中药学]