内源性雌、孕激素与人乳头瘤病毒在宫颈癌发生中的协同作用研究  

Analysis on synergistic action between estrogen,progesterone and human papillomaviruses in cervical cancer

作　　者：王金桃[1] 高尔生[2] 程玉英[3] 闫建文[3] 丁玲[1] 

机构地区：[1]山西医科大学流行病学教研室,太原030001 [2]上海市计划生育科研所 [3]山西省肿瘤医院

出　　处：《中华流行病学杂志》2005年第5期370-373,共4页Chinese Journal of Epidemiology

基　　金：山西省科技攻关课题资助项目 (0 2 10 80 )

摘　　要：目的探讨雌、孕激素对宫颈癌发生的作用及其与人乳头瘤病毒(HPV)的协同作用。方法采用热启动PCR和ELISA法,对141例宫颈癌和129名健康妇女进行了HPVs、HPV16、雌二醇(E2)和孕酮(P)含量的测定。结果宫颈癌组HPVs和HPV16的阳性率分别为75.2%和46.8%,显著高于健康对照组;血清中E2和P的平均浓度均为病例组高于对照组,差异有统计学意义,特别是E2水平与宫颈癌之间呈剂量反应关系;E2与HPVs之间呈正相加交互作用,且有统计学意义。结论HPV感染,特别是HPV16感染在宫颈癌发生中起重要作用;内源性雌激素的升高可增加宫颈癌发生的危险性;高水平的雌二醇和HPV感染对宫颈癌的发生可能具有协同作用。Objective To explore the effects of estrogen (E_2) and progesterone (P) on cervical cancer and the synergistic action between estrogen,progesterone and human papillomaviruses (HPV). Methods Hoted-start polymerase chain reaction (HS-PCR) was used to detect HPVs, HPV16 and ELISA was used to assay E_2 and P on 141 cases with cervical cancer and on 129 healthy controls. Results Positive rates of HPVs and HPV16 were 75.2 % and 46.8 % respectively in cervical cancer group, significantly higher than that in controls. Levels of estrogen and progesterone in case group were significantly higher than that in controls and a dose-responded relationship between the levels of estrogen and cervical cancer was revealed. Estrogen and HPV showed an additive interaction in the development of cervical cancer. Conclusion HPV16 infection played a principal role in the development of cervical cancer. The high levels of entogenous estrogen could increase the risk of cervical cancer and might serve as a cofactor in the development of HPV-induced cervical cancer.

关 键 词：宫颈癌发生 人乳头瘤病毒 孕激素 作用研究 雌二醇(E2) HPV16感染 HPV感染 ELISA法 HPVs 剂量反应关系 内源性雌激素 协同作用 孕酮(P) 健康对照组 健康妇女 平均浓度 交互作用 统计学 PCR 热启动 阳性率 病例组 高水平 

分 类 号：R737.33[医药卫生—肿瘤]