阿尔采默病患者皮肤α-微管蛋白的免疫细胞化学与胶体金免疫电镜观察  

The observe of α-tubulin in skin of Alzheimer’s disease patients with immunocytochemistry and immunoelectron microscopy

作　　者：查彩慧[1] 郭国庆[2] 肖计划[3] 

机构地区：[1]广州市儿童医院儿保科,广州510120 [2]暨南大学医学院解剖学教研室 [3]暨南大学医学院精神病学教研室

出　　处：《中国行为医学科学》2005年第5期388-390,共3页Chinese Journal of Behavioral Medical Science

基　　金：国家自然科学基金资助项目(39570267)

摘　　要：目的观察阿尔采默病(AD)患者皮肤基底细胞α微管蛋白的变化。方法取患者背部皮肤,用免疫细胞化学和胶体金免疫电镜技术观察,并进行定量分析。结果免疫细胞化学染色显示,对照组皮肤免疫阳性染色主要位于表皮层基底细胞的胞质内,棘细胞层和颗粒层可见少量的阳性细胞;AD组基底细胞内着色深,棘细胞层和颗粒层亦有表达,但数量少。胶体金标记结果显示,对照组基底细胞可见较丰富的微管,标记的胶体金散在于胞质。AD组微管较对照组减少;标记的金颗粒数量较对照组明显增多。定量结果显示,AD组α微管蛋白免疫细胞化学染色的AOD值(1. 165±0. 079)、VIOD值(186. 000±15. 853)均较对照组(0. 814±0. 152, 96. 020±14. 755)明显增高(P<0. 01);α微管蛋白胶体金标记颗粒数目AD组(20. 74±3. 99)亦较对照组(15. 50±3. 41)增多明显(P<0. 01)。结论AD患者皮肤基底细胞内微管数量的减少,结构破坏,对皮肤组织的观察可能间接反映神经组织的改变。ObjectiveTo observe changes of α-tubulin in skin from Alzheimer’s disease patients. MethodsThe skins of back from 7 Alzheimer's disease patients were investigated by immunocytochemistry and immunoelectron microscopy, and the results were quantitatively analyzed. Results The results in immunocytochemistry showed that the immunopositve staining were located in plasma of basal cells in control group.Only a few immunopositive cells were found in prickle cell layer and granular layer.The staining of immunopositive cells was more intensive in the skin of Alzheimer's disease patients than that of control group. Although there were some immunopositive cells in prickle cell layer and granular layer, the number of them was less. The results of immunoelectron microscope displayed that the plasma of basal cells had more microtubules, and the number of colloidal golds was dispersed in plasma. Compared with the control group, the number of microtubules was decreased, while the number of colloidal golds was increased significantly. Quantitative results showed that AOD and VIOD in skin of Alzheimer's disease patients(1.165±0.079, 186.000±15.853) were higher than these in control group(P<0.01). There were more colloidal golds in Alzheimer's disease patients(20.74±3.99) than in the control group(P<0.01). ConclusionsThe construction of microtubules in skin of Alzheimer's disease patients was destructed and their quantity was decreased, these changes may reflect indirectly that of nervous tissues.

关 键 词：Α-微管蛋白 阿尔采默病 患者皮肤 免疫电镜观察 免疫细胞化学染色 皮肤基底细胞 免疫电镜技术 棘细胞层 对照组 胶体金标记 定量分析 阳性染色 皮肤免疫 阳性细胞 标记结果 神经组织 皮肤组织 颗粒层 细胞内 颗粒数 AD 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R197.323[医药卫生—临床医学]