冲突性应激致大鼠行为和内分泌的变化(英文)  

Behavioral and endocrine changes following conflict-related stress

作　　者：吴万振[1] 陈夫银[1] 张永东[1] 赵虎[1] 

机构地区：[1]汕头大学医学院法医学教研室

出　　处：《中国行为医学科学》2005年第5期391-393,共3页Chinese Journal of Behavioral Medical Science

基　　金：国家自然科学基金(30371578);广东省自然科学基金(021219)~~

摘　　要：目的研究在冲突性应激条件下大鼠行为和内分泌的变化。方法56只成年雄性SD大鼠,随机分为8组,每组7只,利用Vogel冲突实验方法稍加修改,作为应激源,开场试验检测行为反应,测量体重,利用酶联免疫方法检测血清中皮质酮的含量。结果与对照组相比, 1周、2周、4周应激组大鼠表现为体重明显降低(216. 5±8. 71g, 194. 94±14. 82g, 259. 75±13. 83g):;在运动性方面, 1、2、4周应激组大鼠(67. 1±9. 2, 53. 66±6. 86, 59. 25±6. 46)有增加的趋势或者明显增加,在实验过程中,所有应激组大鼠皮质酮不同(1次应激组274. 41±25. 39ng.ml-1, 1周应激组240. 44±29. 51ng.ml-1, 2周应激组254. 21±37. 16ng.ml-1, 4周应激组161. 02±46ng.ml-1 )较对照组(89. 33±14. 04ng.ml-1 )明显升高。结论在应激大鼠表现的增多的运动,升高的皮质酮以及降低的体重,与焦虑障碍的某些特点相似,并且行为和内分泌的变化可以维持一段时间。冲突相关的应激可以作为研究焦虑及其他精神创伤应激比较理想的动物模型。ObjectiveTo investigate behavioral and endocrinal changes following the conflict-related stress. Method Male Sprague-Dawley rats were exposed to stressors using Vogel conflict test. Body weight and behavioral responses in open field test were investigated during the whole procedure. Serum corticosterone level was analyzed using immunoassay. ResultsCompared with the control, the rats of the 1-week, 2-week and 4-week stressed group showed significantly decreased body weight. There was increasing tendency or significant difference in locomotion between the 1-week, 2-week and 4-week stressed group and their control. And the hormone levels for the stressed animal were reliably high. ConclusionsThe increased locomotion, and high corticosterone level and reduced body weight in stress animal,was consistent with the features of anxiety-like disorders. Furthermore, the behavioral and endocrine changes produced by conflict-related stress could maintain a long time. The conflict-related stress paradigm may be used as psychological stress animal modein the future.

关 键 词：大鼠行为 内分泌 冲突 酶联免疫方法 Vogel 皮质酮 应激条件 SD大鼠 实验方法 行为反应 开场试验 实验过程 应激大鼠 焦虑障碍 动物模型 创伤应激 对照组 应激源 体重 运动性 检测 升高 

分 类 号：R363[医药卫生—病理学]