细胞间粘附分子1在卵巢肿瘤的表达及其对癌细胞粘附侵袭能力的影响  

Expression of Intercellular Adhesion Molecule 1 and its Effects on Adhesion and Invasion of Ovarian Cancer

作　　者：刘鸣[1] 韦德英[1] 汤春生[1] 

机构地区：[1]山东省立医院,山东济南250021

出　　处：《实用妇产科杂志》2005年第5期279-281,i001,共4页Journal of Practical Obstetrics and Gynecology

基　　金：山东省优秀中青年科学家科研奖励基金项目(编号:01BS56)

摘　　要：目的探讨卵巢癌组织及细胞株细胞间粘附分子1(ICAM1)的表达及其意义,探讨ICAM1对卵巢癌细胞粘附侵袭能力的影响。方法分别用免疫组化方法及流式细胞术检测卵巢上皮性肿瘤组织及卵巢癌细胞ICAM1表达,通过MTT法细胞粘附实验和Boyden小室法体外侵袭实验检测ICAM1在卵巢癌细胞粘附侵袭过程中的作用。结果上皮性卵巢癌组织ICAM1阳性表达率71.8%(28/39)明显高于良性肿瘤组织40%(8/20)。SKOV3细胞高侵袭克隆SKOV3S1ICAM1荧光强度高于低侵袭细胞克隆SKOV3-S21。ICAM1抗体对SKOV3S1细胞粘附及侵袭人工基底膜能力的抑制率分别为41.9%和55.0%。结论ICAM1在上皮性卵巢癌侵袭转移过程中起重要作用。Objective: To investigate the expression of intercellular adhesion molecule 1 in ovarian cancer and its role in adhesion and invasion of ovarian cancer cells. Methods: The expression of ICAM-1 in epithelial ovarian neoplasms and subclones of SKOV3 cells was determined by SP immunohistochemical staining and FCM. The role of ICAM-1 in adhesion and invasion of ovarian cancer cells was observed with cell adhesion test and Boyden Chamber invasive assay respectively. Results: The expression of ICAM-1 was 71.8%(28/39)in epithelial ovarian cancer mass and 40%(8/20) in benign mass . The fluorescent intensity of SKOV3-S1, the highly invasive clone of SKOV3, was higher than the noninvasive clone SKOV3-S21. The antibody of ICAM-1 can significantly inhibits the SKOV3-S1 cells adhere to and invade the basement membrane matrix by 41.9% and 55.0% respectively. Conclusions: ICAM-1 plays an important role in the adhesion and invasion of ovarian cancer.

关 键 词：卵巢肿瘤 细胞间粘附分子1 肿瘤侵袭 

分 类 号：R737.31[医药卫生—肿瘤]