参麦注射液对大鼠脑出血后迟发性神经细胞损伤的保护作用  

Protective effects of Shenmai injection on the delayed injury of the cerebral neurons in rat induced by intracerebral hemorrhage

作　　者：何泽云[1] 李晓峰[2] 屈波[2] 

机构地区：[1]浙江大学医学院第一附属医院博士后流动站,浙江杭州310003 [2]湖南中医学院,湖南长沙410007

出　　处：《中国中药杂志》2005年第7期526-530,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金(39900199);湖南省自然科学基金(99JJY2003);中国博士后基金(2003034521)

摘　　要：目的:观察参麦注射液对大鼠脑出血后迟发性神经细胞损伤的保护作用。方法:采用Rosenberg法复制大鼠脑出血模型,观察脑出血后大鼠海马CA1区神经细胞病理形态结构、神经细胞线粒体DNA缺失、C-myc基因、PDGFA基因表达及参麦注射液的防治作用。结果:参麦注射液减少大鼠脑出血后海马CA1区锥体细胞凋亡数目,抑制血肿周围脑组织线粒体DNA片段缺失,参麦注射液对出血早期C-mycmRNA的表达无明显影响,但可使PDGFAmRNA表达明显下调并时限延长。结论:参麦注射液可能通过调节PDGF表达对脑出血后迟发性神经细胞损伤修复有保护作用。Objective: To observe protective effects of Shenmai (SM)injection on the delayed injury of the cerebral neurons in rat with intracerebral hemorrhage. Method: Rosenberg models of intracerebral hemorrhage was established and the effects of SM injection on the pathologic changes in neuronal structure, mitochondria-DNA(mtDNA)deletion, C-myc gene and expression PDGF-A gene in hippocampal CA 1 areas, were investigated. Result: SM injection inhibited the apoptosis of pyramidal cells in the hippocampal CA 1 areas, and decreased the degree of mtDNA deletion in the neurons in the injured area. SM injection had no effect on gene expression of C-myc at initial stage a intracerebral hemorrhage, but significantiy decreased the level of PDGF-A mRNA and prolonged the time of its expression. Conclusion: SM injection might attenuate the delayed injury induced by intracerebral hemorrhage via regulating the expression of PDGF.

关 键 词：参麦注射液 神经细胞损伤 保护作用 迟发性 大鼠 线粒体DNA缺失 海马CA1区 C-myc基因 DNA片段缺失 脑组织线粒体 PDGF-A mRNA表达 脑出血模型 基因表达及 形态结构 细胞病理 防治作用 细胞凋亡 血肿周围 损伤修复 

分 类 号：R285.5[医药卫生—中药学]